Supplementary data: (a) (b) (c) 10 nm 10 nm 10 nm 100 nm 100 nm 100 nm Fig. 1STEM images of Ag nanoparticles prepared at different CDT concentrations: (a) CDT-Ag1; (b) CDT-Ag2 ; and (c) CDT-Ag3. The insets show higher magnification images. Furuzono et al.
Supplementary data: 0 h 2.0 h Time 0.5 h 1.0 h Dilution ① ② ③ ④ ⑤ ~ ⑧ ① ② ③ ④ ⑤ ~ ⑧ ① ② ③ ④ ⑤ ~ ⑧ ① ② ③ ④ ⑤ ~ ⑧ Control CDT-Ag1 CDT-Ag2 CDT-Ag3 Free-CDT Fig. 2S Antimicrobial activity of Ag nanoparticles coated with different concentrations of CDT against Escherichia coli.After mixing E. coli and Ag nanoparticles, indicated by CDT-Ag1-3, 20 µl of reactions were sampled at 0-2 hours shown at the top of each photo.Control means no addition of Ag materials, and free CDT means addition of CDT regent without Ag. Samples and seven ten-fold serial dilutions are illustrated by numbers ①～⑧, for instance, each sample shown by ① should contain 1.4 x 103E. coli cells if no antimicrobial activities were presented. Furuzono et al.
Supplementary data: toluene toluene toluene toluene Ag+ Ag+ Ag+ Ag+ Ag+ Ag+ Ag+ Ag+ Ag+ Ag+ Ag+ Ag+ Ag+ Ag+ Ag+ Ag+ NaBH4 S S S S S S S S S S COOH COOH COOH COOH COOH COOH COOH COOH COOH COOH water water water water Agions were reduced to metallic Ag. Ag ions in water phase were transferred into the oil phase by TOAB. CDTs were bonded with Ag ions. The solution mixtures were separated into two phases. Separation, evaporation and purification HOOC HOOC HOOC S S S TOAB CDT Ag ion Ag Ag HOOC HOOC HOOC S S S S S S S S COOH COOH COOH COOH COOH CDT-Ag nanoparticle COOH COOH COOH COOH COOH S S S S S Fig. 3S Schematic presentation of the synthesis mechanism of CDT-Ag nanoparticles by two-phase reduction system. Furuzono et al.