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Restriction Enzymes & Gel Electrophoresis

Restriction Enzymes & Gel Electrophoresis. Restriction Enzymes. Cut DNA into pieces Recognize and cut at specific sites along the DNA molecule. Example 1:. Enzyme EcoRI reads GAATTC and cuts between the G and A. If the DNA strand reads: A A G A A T T C T C G A A T T C G G A

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Restriction Enzymes & Gel Electrophoresis

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  1. Restriction Enzymes & Gel Electrophoresis

  2. Restriction Enzymes Cut DNA into pieces Recognize and cut at specific sites along the DNA molecule.

  3. Example 1: Enzyme EcoRI reads GAATTC and cuts between the G and A. If the DNA strand reads: A A G A A T T C T C G A A T T C G G A A A G / A A T T C T C G / A A T T C G G A How many fragments are there? 3 How many bases in each fragment? A A G / A A T T C T C G / A A T T C G G A 3 8 8

  4. Example 2: Enzyme HindIII reads AAGCTT and cuts between the A's. If the DNA strand reads: A A G C T T C G A A G C T T A G A / A G C T T C G A / A G C T T A G How many fragments are there? 3 How many bases in each fragment? A / A G C T T C G A / A G C T T A G 1 8 7

  5. Gel Electrophoresis A process used to compare different DNA's. For example, can be used in paternity tests or forensics. Restriction Enzymes help in the process of gel electrophoresis.

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