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Immunoglobulin Gene Rearrangement

Immunoglobulin Gene Rearrangement. Lu Tian Deb Walter. Immunoglobulin. A Y-shaped protein produced by B cells that is used by the immune system to identify and neutralize foreign objects (bacteria and viruses).

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Immunoglobulin Gene Rearrangement

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  1. Immunoglobulin Gene Rearrangement Lu Tian Deb Walter

  2. Immunoglobulin • A Y-shaped protein produced by B cells that is used by the immune system to identify and neutralize foreign objects (bacteria and viruses). • Each antibody contains a paratope that is specific for one particular epitope on an antigen, allowing these two structures to bind together. http://en.wikipedia.org/wiki/Antibody

  3. Isotypes • IgA • IgD • IgE • IgG • IgM http://en.wikipedia.org/wiki/File:Mono-und-Polymere.svg

  4. Structure of Immunoglobulin http://www.ebioscience.com/knowledge-center/antigen/immunoglobulin/structure.htm

  5. Immunoglobulin diversity 1 Domain variability 2V(D)J recombination 3 Somatic hypermutation and affinity maturation 4 Class switching 5 Affinity designations

  6. Immunoglobulin Gene Rearrangements • The ability of cells of the immune system to make antibodies requires multiple programmed rearrangementsof immunoglobulin genes. • Randomly combines Variable, Diverse, and Joining gene segments in vertebrate lymphocytes. • Because of its randomness in choosing different genes, is able to diversely encode proteins to match antigens .

  7. Rearrangement in Heavy Chain V, D, J First, D-J recombination followed by the joining of V gene segment, forming a rearranged VDJ gene segment. Any DNA between these gene segments is deleted http://en.wikipedia.org/wiki/V(D)J_recombination

  8. Rearrangement in Light Chain Two types of chains: The kappa (κ) and lambda (λ) chains, but Only V and J the immunoglobulin rearrange in a similar way http://pathmicro.med.sc.edu/mayer/gen-2.jpg

  9. Germline Immunoglobulin DNA Jung et al., Annual review of immunology 24 (2006)

  10. V(D)J Recombination and 12/23 Rule Tonegawa, T. Nature 302,5909 (1983)

  11. RAG Mediated DNA Rearrangements Bassinget al. Cell 109 (2002)

  12. Hairpin Opening and Artemis Protein Ma et al. Cell Press 108 (2002)

  13. V(D)J Joining Murphy, Geha and Notarangelo. Immuno Biology 8th edition (2011)

  14. Review of V(D)J Joining Bassinget al. Cell 109 (2002)

  15. Regulation of Further Recombination Jung et al., Annual review of immunology 24 (2006)

  16. Somatic Hypermutation Jacobs and Bross. Curent opinion in immunology. 13 (2001)

  17. Class Switch Recombination Maniset al. Trends in immunology. 23, 1 (2002)

  18. Severe Combined Immunodeficiency (SCID) Mice Jackson Laboratories <jax.org>

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