Laboratory Safety Laboratory Skills p1024-1027

1. Laboratory Safety Laboratory Skills p1024-1027

2. Objectives TEKS / TAKS 1A The student will demonstrate safe practices during field and laboratory investigation.

3. Safety Rules • NO food or drink in the lab. • NO horseplay or loud voices in the lab. • Keep workstation neat and clean. • Understand what you are doing in the lab before beginning the lab experiment. • Keep hands away from face while working. • Know emergency procedures in case of an emergency. • Work with a partner, but produce your OWN work. • Wash your hands before leaving the lab.

4. General Guidelines for Lab Safety • Only perform experiments specifically assigned by your teacher. • Familiarize yourself with the lab and any safety precautions before entering the lab. • Tie back hair, roll up sleeves, put on and leave on, any required safety protective equipment for each lab. • No contact lens are allowed in the lab.

5. General Guidelines for Lab Safety • Know the location of all safety and emergency equipment used in the laboratory. • Immediately report and accident, incident, or hazard – no matter how trivial –to teacher. • In case of fire, alert your teacher and leave the lab following given procedure. • Do not eat or drink in the lab.

6. General Guidelines for Lab Safety • Do not fool around or horseplay in the lab. • Do not apply cosmetics in the lab. • Keep your work area neat and uncluttered. • Clean your work area at the end of each lab period. • Wash your hands with soap and water after each lab.

7. Eye Safety • WEAR SAFETY GOGGLES as directed. • Do not wear contact lenses in the lab • Chemicals can splash onto the contact causing serious, permanent eye damage. • Do not look directly at the sun through any optical device or lens, and do not reflect direct sunlight to illuminate a microscope. • This concentrates light rays to an intensity that can cause blindness. Emergency Eye Wash Station Immediately flush eyes with running water for 15 minutes. Hold eyes open with fingers to get under lid. Have lab partner notify teacher immediately.

8. Hand Safety • Do not cut objects while holding them in your hand. • Put the specimen in a dissecting pan or on appropriate lab table covering. • Always draw the scalpel or cutting tool a yourself to avoid injuring those around you. • Wear protective gloves when working with an open flame, chemical, solution, or wild or unknown plants.

9. Safety with Gases or Inhalants Should have used a fume hood! • Do not inhale any gas or vapor unless directed to do so by your teacher. • WAFT any scent to your nostrils ensure that you do not destroy the mucus membranes of your respiratory system. • Handle materials that emit vapors or gases in a well ventilated area. • Use the chemical fume hood as specified in the lab or if there is any question of the fumes.

10. Sharp-Object Safety • Use extreme care when handling all sharp and pointed instruments, such as scalpels, sharp probes, and knives. • Do not use double-edged razor blades in the laboratory. • Do not cut objects while holding them in your hand. • Cut objects on a suitable work surface. Always cut in a direction away from your body.

11. Animal Care and Safety • Do not approach or touch any wild animals. • Always get your teacher’s permission before bringing any animal (including pets) into the school building. • Handle animals only as directed by your teacher. Mishandling or abusing any animal will not be tolerated.

12. Heating Safety • Be aware of flames, sparks, or heat before working with flammable materials. • When heating chemicals or solutions in a test tube, do not point the test tube toward anyone. • Avoid using open flames • Know the location and use of the fire extinguisher and fire-safety blankets. • Use tongs or insulated holders when handling heated objects.

13. Hygienic Care • Keep hands away from your face and mouth while working in the lab. • Wash your hands before leaving the lab. • Use proper technique when handling bacteria or other microorganisms. These are pathogens!!! Keep petri dishes sealed. Dispose of petri dishes as instructed by teacher for proper disposal. • Wash hands after touching any cultures dish! Spill of caustic material on clothing • Get to safety shower ASAP. Remove all clothing and rinse 15 minutes. Have partner inform teacher. Permanent skin damage can occur from chemical spills.

14. Glassware Safety • Inspect glassware for chips or cracks BEFORE using. • Use gloves and glycerin to insert glass tubing into a stopper. • Notify teacher immediately if glassware breaks. Do not attempt to pick it up with hands. • Dispose of glass in specified container.

15. Proper Waste Disposal • Clean and Sanitize all work surfaces and safety attire after each lab period. • Dispose of all sharp objects and contaminated materials as directed by your teacher.

16. Electrical Safety • Do not use equipment with frayed electrical cords or loose plugs.  • Fasten electrical cords to work surface to prevent trips and falls. • Do not use electrical equipment near water. • Hold the rubber head of the cord when plugging and unplugging.

17. Clothing Protection • Wear and apron or laboratory coat at all times in the laboratory to prevent chemicals from contacting skin or clothes.

18. Plant Safety • Do not eat any plant part used in a lab. • Wear disposable gloves when handling plants. • Wash hands thoroughly after handling any plant. Avoid touching face or eyes. • Do not pick wildflowers or other plants unless direct by your teacher.

19. Chemical Safety • Always wear safety goggles, gloves, and a lab apron or coat when working with any chemicals. • Do not touch, taste, or smell any chemicals unless instructed by your teacher. • Know location of eye wash and shower stations. • Do not mix any chemicals unless instructed to do so by your teacher. • Do not pour water into a strong acid or base. • Report any spill immediately to your teacher. • Never return chemicals to the original container….wait for teacher instructions.

20. Safety SymbolRates Hazards from 0-4 • Red • Fire Hazard • Yellow • Reactivity Hazard • Blue • Health Risk • White • Radioactivity 1 3 0 0