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Understanding Temperature Classifications of Microorganisms: Psychrophiles to Thermophiles

This guide explores the classifications of microorganisms based on temperature preferences. It discusses the three main types: Psychrophiles (cold-loving, 0-20°C), Mesophiles (moderate temperature-loving, 20-40°C), and Thermophiles (heat-loving, 40-100°C). The concept of Thermal Death Point (TDP) is also introduced, defining the temperature at which all bacteria, along with their endospores, are killed within 10 minutes. Using various bacterial samples, we set specific hot plate temperatures to observe growth and survival rates, illuminating the adaptations of these microorganisms to extreme environments.

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Understanding Temperature Classifications of Microorganisms: Psychrophiles to Thermophiles

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  1. Ex. 18 Temperature

  2. Temperature Classifications • 1. Psychrophiles • 2. Mesophiles • 3. Thermophiles • “Cold loving” • 0 – 20 degrees C • “moderate temp loving” • 20 – 40 degrees C • “heat loving” • 40 – 100 degrees C

  3. Temperature Classifications 4 c 37 c 55 c

  4. Thermal Death Point (TDP) • Temperature in which all bacteria and their endospores (if they have them) are killed within 10 minutes.

  5. Add .5ml bacteria to Test Tubes 40 60 80 100 40 60 80 100 E. coli Staph. epi 40 60 80 100 40 60 80 100 Micrococcus luteus Bacillus stearothermophilus

  6. Set Hot Plates at desired temperatures 40 60 80 100

  7. 40 60 80 100 E. coli Staph. epi Micrococcus luteus Bacillus stearothermophilus

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