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This study investigates Spiralin expression in Mcc strains using immunoblot analysis with anti-Spiralin antibodies. Mycoplasma cells were probed with specific antibodies on a nitrocellulose membrane.
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a b Figure S3. Expression of spiralin in Mccusing pMyBK1 derivatives. Wholecell dot immunoblot of 12 Mcc transformants harboring the spiralin expression vector pCM-K3-spi (a) or the emptyvector pCM-K3 (b). Mycoplasma cellswereapplied to a nitrocellulose membrane and probedwithrabbit anti-spiralin antibodies and anti-rabbitIgGperoxidaseconjugate.