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PKC and Memory

PKC and Memory. Manhasset Science Research Paris Thatos. Need. Long-term trend in drug-related deaths. Percentage of People Experiencing a Mental Disorder in their Lives. (Gotz, 2004). (Gallaher, 2007). Long Term Potentiation. The complex chemical processes that form long-term

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PKC and Memory

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  1. PKC and Memory Manhasset Science Research Paris Thatos

  2. Need Long-term trend in drug-related deaths Percentage of People Experiencing a Mental Disorder in their Lives (Gotz, 2004) (Gallaher, 2007)

  3. Long Term Potentiation • The complex chemical processes that form long-term memories (Cook, 2006) (Kanehisa, 1999)

  4. PKC (Protein Kinase C) • Has many isoforms (PKMzeta,PKMa) (Abel, 2004)

  5. Olfactory Memory Capacity of the Cricket Gryllus Bimaculatus- • Yukihisa Matsumoto • Pubmed

  6. PKMzeta maintains spatial, instrumental and classically conditioned Long term memories • Peter Serano, Todd C. Sacktor • PLoS Biology (Morris, 2004)

  7. Lit Review

  8. Lit Review

  9. Purpose/Hypothesis • The purpose of this experiment is to see whether Bisindolymaleide disrupts long term memories in Gryllus Bimaculatus when treated with it pre- and post- conditioning • It is hypothesized that with all administeringings of Bisindolymaleide,

  10. Grasshopper (Gryllus Bimaculatus) n=60 Group 1 - Fed normal food n=20 Group 2 - Fed normal food n=20 Group 2 - Fed food mixed with 1 mg Bisindolymaleide n=20 Each Group trained to associate one scent with reward and one with punishment Group Two now exposed to Bisindolymaleide in water supply Placed in container with two conditioned scents on either end (reward/punishment). Time spent on each scent is recorded Statistiacaly aanalyzed with a One-way ANOVA followed by a Scheffe post-hoc test (p<o.o5)

  11. Protocol

  12. Budget Total = $133.70

  13. Do-ability • Materials already stocked in lab: Plastic Syringe Water Sodium Chloride Pyrex Beaker

  14. Bibliography • Olton, D.S., & Samuelson, R.J. (1976). Remembrance of places passed: Spatial memory in rats. Journal of Experimental Psychology: Animal Behavior Processes, 2, 97-116. • Ling D, Benardo L, Serrano P, Blace N, Kelly M, Crary J, Sacktor T (2002). "Protein kinase Mzeta is necessary and sufficient for LTP maintenance". Nat. Neurosci.5 (4): 295-6. • Serrano P, Yao Y, Sacktor T (2005). "Persistent phosphorylation by protein kinase Mzeta maintains late-phase long-term potentiation". J Neurosci25 (8): 1979-84. • Pastalkova E, Serrano P, Pinkhasova D, Wallace E, Fenton A, Sacktor T (2006). "Storage of spatial information by the maintenance mechanism of LTP". Science313 (5790): 1141-4 • Cooke SF, Bliss TV (2006). "Plasticity in the human central nervous system". Brain129 (Pt 7): 1659-73 • Boron, Walter F. (2005). Medical Physiology: A Cellular And Molecular Approach. Elsevier/Saunder • Clugnet, MC; LeDoux JE (01 Aug 1990). "Synaptic plasticity in fear conditioning circuits: induction of LTP in the lateral nucleus of the amygdala by stimulation of the medial geniculate body." (PDF). J Neurosci10 (8): 2818-24. • Sweatt J (1999). "Toward a molecular explanation for long-term potentiation". Learn Mem6 (5): 399-416.

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