1 / 17

Quorum Sensing

Quorum Sensing. By Sachi Lagwankar. What is QS?. process of bacterial cells “talking” to each other in order to be informed of the population density in its vicinity Bacteria can only act on the host cell when the impact of the process is at its maximum Mutants (change signaling patterns)

bryson
Télécharger la présentation

Quorum Sensing

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Quorum Sensing By Sachi Lagwankar

  2. What is QS? • process of bacterial cells “talking” to each other in order to be informed of the population density in its vicinity • Bacteria can only act on the host cell when the impact of the process is at its maximum • Mutants (change signaling patterns) use QS in order to attack. • used commonly when starting infections in organisms.

  3. Autoinducers Gram positive bacteria Gram negative bacteria Pseudomonas quinolone signal (PQS; 2-heptyl-3-hydroxy-4-quinolone) Oligopeptide signal

  4. What are Autoinducers? Chemical molecular signals gram negative bacteria: acylatedhomoserine lactones (AHL) Gram positive bacteria: oligo-peptides

  5. Importance only way mutants know how to communicate scientists are trying to find a way to inhibit this so that mutants don't take over host cells.

  6. Biofilm sticky layer of polymers that microorganisms secrete and stick to one another on all surfaces Controlled by AI 2 Common in people, though they can cause illness

  7. Antibiotics Multidrug-Resistant Tuberculosis (MDR TB) is resistant to the two most commonly used antibiotics for TB- isoniazid (INH) and rifampin (RIF). This means that people who get TB are unable to be cured using these antimicrobials.   “tame” bacteria, and not to kill them

  8. Option 1 Paul Williams: generate a drug that can search and take out bacteria and that will prevent them from doing damage to the host cell.

  9. Option 2 Geoff Watts: stop bacteria from sending signals, eliminate infections

  10. Option 3 Peter Greenberg: encourage mutants to dominate fatal wounds and they do better and do not use quorum sensing and are less virulent

  11. Option 4 Steve Diggle: experiment- add mutants to the potential fatal wounds already infected with the virulent bacteria, those wounds actually did better.

  12. Option 5 Alan Smyth: a natural quorum sensing inhibitor is to use extracts of plants like garlic extract

  13. Design Goal the immune system take over the virus before it attacks the host cell and infects it. when mutants send signal, cell makes protein to be released out of cell confuse the mutant into thinking there are more of it. when the mutant attacks, the human cell will use its immune system to kill the mutant.

  14. Design:

  15. Truth Table

  16. Ethics idea of how quorum sensing can be used to prevent illness in humans. this is designed to help humans and prevent them from getting illnesses.

  17. Sources • http://www.bbc.co.uk/programmes/p01bhlvr • http://rstb.royalsocietypublishing.org/content/362/1483/1241.full • http://downloads.bbc.co.uk/podcasts/worldservice/discovery/discovery_20130708-2000a.mp3 • http://www.ncbi.nlm.nih.gov/pubmed/23142623 • https://www.youtube.com/watch?v=2R4XHRufoWI • http://jb.asm.org/content/187/13/4372 • http://en.wikipedia.org/wiki/Autoinducer • http://www.perspectivesinmedicine.net/content/2/11/a012427.full • http://www.ncbi.nlm.nih.gov/pubmed/11544353 • https://www.bio.cmu.edu/courses/03441/TermPapers/99TermPapers/Quorum/table.htm • http://www.ncbi.nlm.nih.gov/pubmed/11018124 • http://www.niaid.nih.gov/topics/tuberculosis/understanding/whatistb/pages/tbdefinitions.aspx • http://mpkb.org/home/pathogenesis/microbiota/biofilm

More Related