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Practical Microbiology

Practical Microbiology. Ibrahim Mahmoud Sayed Ibrahim. Instrument Instruments 1-Seitz filter 2—MacKnotoch 3-Gas Pack system. 2- IMVC test. IMVC of E.coli.

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Practical Microbiology

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  1. Practical Microbiology Ibrahim Mahmoud Sayed Ibrahim

  2. Instrument Instruments1-Seitz filter 2—MacKnotoch 3-Gas Pack system

  3. 2- IMVC test • IMVC of E.coli

  4. IMVC testCitrobacter Klebsellia

  5. TSI (triple sugar iron) • LF (Ecoli) Pseudomonas Proteus

  6. Christen urea media Simmon Citrate • Pink +ve proteus Green –ve Ecoli • Yellow –ve Blue +ve Klebellia

  7. Sugar fermentation of Salmonella • S.paratyphi B • S.paratyphi A S.typhi

  8. Sugar fermentation of Shigella • Sh.dysentriae Sh.flexenerii Sh.Sonneii

  9. Sugar Fermentation of Neisseria • N.gonorrhea N.meningitidis

  10. Non-inoculated Media • Loffler’s serum Dorset Egg Lowstein Jensen media

  11. Non inoculated media TCBS Anaerobic media Thioglycolate –Robertson cooked meat-Deep agar

  12. II inoculated mediaNutrient Agar Staph (endopigment) Pseudomonas (Exopigment) Proteus (swarming) Sabroud dextrose (yeast)

  13. II-Blood agar • Blood agar (B-hemolysis) as Staph aureus, Strept Pyogens • Blood tellurite as Corynebacterium diphtheriae

  14. III Chocholate agar • Chocholate agar Optochin sensitivity • Pneumococci +ve zone : Pneumococci • -ve zone : S.viridans

  15. IV) Pink Plate • Mannitol salt agar MacConkey Agar • Staph aureus Lf (Ecoli)

  16. V) EMB plate • E.coli Klebsellia

  17. VI) Special Plate • MacConkey Agar Sabroud dextrose Agar • NLF(Salmonella) Mould

  18. VI) Special Plate • Milk Agar Bismuth Sulphite • Staph Salmonella

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