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Microbiology Practical I

Microbiology Practical I. The following slides were contributed by your fellow BIO 251 students…. Use side “ ” key to view slides…. Lab 1: Eukaryotes and Microscopy. Know the parts of the microscope…. Lab 1: Eukaryotes and Microscopy. Paramecium caudatum. Lab 1: Eukaryotes and Microscopy.

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Microbiology Practical I

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  1. Microbiology Practical I The following slides were contributed by your fellow BIO 251 students… Use side “” key to view slides…

  2. Lab 1: Eukaryotes and Microscopy Know the parts of the microscope…

  3. Lab 1: Eukaryotes and Microscopy Paramecium caudatum

  4. Lab 1: Eukaryotes and Microscopy Euglena gracilis

  5. Lab 1: Eukaryotes and Microscopy Amoeba proteus

  6. Lab 1: Eukaryotes and Microscopy Fungus – Saccharomyces cerevisiae

  7. Lab 1: Eukaryotes and Microscopy Cyanobacteria ~ Nostoc

  8. ~Bacteria slides~ Micrococcus luteus

  9. ~Bacteria Slides~ Bacillus subtilis

  10. ~Bacteria Slides~ Bacillus anthracis

  11. ~Bacteria Slides~ Rhodospirillum rubrum Sorry not in focus…

  12. ~Bacteria Slides~ See other file for Gram Stain, Endospore, Acidfast, and Capsule Staining… [ Adobe Acrobat of Gram and Endospore ] Some Endospore Staining ~

  13. Streak Plate: Know colony characteristics (color, elevation, margin, etc.)

  14. Spread Plate vs. Streak Plate:

  15. Streak Plate:

  16. Spread Plate: Mixed – Bacillus subtilis & Serratia marcescens

  17. Practical I Unknown Tests: ~ Simple Stain ~ Gram Stain ~ Endospore Stain ~ Acid-Fast Stain (Demo) ~ Capsule Stain (Demo) ~ Motility (Hanging Drop, Wet Mount, Soft Agar Deep) ~ Obligate Aerobe, Facultative Anaerobe, Aerotolerant Anaerobes, Microaerophiles (Gas Pak, Eugon Agar, Thioglycollate Broth)

  18. Gas Pak Anaerobic vs. Aerobic:

  19. Dilution Problems:

  20. Dilution Problems:

  21. Bacterial Growth Curve: Make sure you know how to: Use a spectrophotometer to measure absorbance. Produce a log graph of the calculations. Calculate generation time from a growth curve.

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