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MICROBIOLOGY PRACTICAL

MICROBIOLOGY PRACTICAL. Reproductive block 2013. Sexually Transmitted Diseases Practical. Objectives. Name various etiological agents causing sexually transmitted diseases (STD) Describe the clinical presentations of STD (ie Gonococcal vs non-gonococcal)

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MICROBIOLOGY PRACTICAL

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  1. MICROBIOLOGYPRACTICAL Reproductive block 2013

  2. Sexually Transmitted Diseases Practical

  3. Objectives • Name various etiological agents causing sexually transmitted diseases (STD) • Describe the clinical presentations of STD (ie Gonococcal vs non-gonococcal) • Describe the proper specimen collection for diagnosis of STD • Discuss the microbiological and non microbiological methods for diagnosis of STD • Outline the management , complication and prevention of STD

  4. Case 1 • A 23-year-old alcoholic and drugs (cocaine) addict single male arrived from his trip to South East Asia six months ago. He gave history of multiple sexual partners. Two months ago he developed ulcer on his penis which disappeared compeletly. A full physical notes a rash on both his palms and his soles

  5. What are the possible causes for his presentation? • Treponema Pallidum • Herpes Simplex Virus 2 • Haemophilus Ducreyi

  6. Genital ulcer

  7. How could you differentiate between them based on s/s of the patient?

  8. How could you differentiate between them based on s/s of the patient?

  9. What investigations would you like to order for him? Explain how those investigations would help you?

  10. What investigations would you like to order for him? Explain how those investigations would help you?

  11. The lesion was sampled and examined by dark-field microscopy;

  12. Gram stain; gm-ve small bacilli Haemophilus Ducreyi Cytopathic effect of HSV in cell culture

  13. Case 2 • A 35-year-old Pilipino married male presented to the emergency room complaining of dysuria for the last 24-hour and noted some "pus-like" drainage in his underwear and the tip of his penis.

  14. "pus-like" drainage

  15. Base on the finding, what is the most likely diagnosis? Briefly outline the management of this patient?

  16. What are the possible causes for his presentation?

  17. What are the possible causes for his presentation?

  18. What investigations do you like to order for him? Explain how those investigations would help you?

  19. What investigations do you like to order for him? Explain how those investigations would help you?

  20. Gram Stain of Urethral Swab

  21. Base on the finding, what is the most likely diagnosis? Briefly outline the management of this patient?

  22. Case 3 • A 24-year-old female noted vaginal itching and irritation with a discharge. Previously, she developed a yeast infection that was treated with over-the-counter medications and resolved. Thinking that this was recurrence, she again self-treated. This time, however, the symptoms did not resolve.

  23. What are the possible causes for her presentation? • Bacterial vaginosis • Candida vaginitis • Trichomonas vaginalis • Allergic vaginitis • Chlamydia trachomatis • Neisseria gonorrhoeae

  24. What investigations would you like to order for her? Explain how those investigations would help you?

  25. What investigations you like to order for her? Explain how those investigations would help you?

  26. Wet prep/Gram stain A wet mount of the swab demonstrates "swimming" Motile Trophozoites.

  27. Base on the finding, what is the most likely diagnosis? Briefly outline the management this case?

  28. What organisms would you screen for in any patient presented with any STD?

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