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Protein Expression under Ca2+ Depletion in Pseudomonas aeruginosa

Investigating protein expression patterns in Pseudomonas aeruginosa under Ca2+ depletion, using ExoT and ExoS as key markers. Manipulating IPTG pulses during depletion to unravel molecular mechanisms. Study conducted on CHA-D1 and PAO1 strains.

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Protein Expression under Ca2+ Depletion in Pseudomonas aeruginosa

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  1. A MW(kDa) 1 2 3 4 5 6 7 8 66 ExoT ExoS 43 14.3 2.3 (1) 2.2 0.5 17.2 7.4 - - - - - IPTG pulse + + + IPTG during Ca2+depletion + CHA-D1 CHA-D1 (pexsAind) CHA PAO1 PAO1 (pexsAind) Strains B MW(kDa) 1 2 3 4 66 ExoT ExoS 43 12.6 4.1 - - IPTG Pulse + + 8H Time laps before Ca2+ depletion 6H

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