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AJ10 Immunoreactivity and Neuronal Degeneration in SOD1 G85R and G93A Mouse Models

This study investigates the relationship between AJ10 immunoreactivity and degenerating motor neurons (MNs) in the ventral horn of lumbar spinal cord sections from end-stage SOD1 G85R and G93A mice. Both fast and slow strain models were examined. Double immunostaining with AJ10 (red) and NeuN (green) reveals that AJ10-positive profiles in degenerating MNs do not co-localize with healthy neuronal cell bodies marked by NeuN. Additionally, we explore the colocalization of AJ10 immunoreactivity with ubiquitin, highlighting the involvement of mutant SOD1 aggregates in neurodegeneration.

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AJ10 Immunoreactivity and Neuronal Degeneration in SOD1 G85R and G93A Mouse Models

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  1. AJ10 NeuNmerge SOD1 G85R (P-340) C A B E D F SOD1 G93A fast (P-136) G H I L J K SOD1 G93A slow (P-247) M O N P Q R AJ10 Ubiquitin merge U S T Supplementary Figure 2. AJ10 immunoreactivity is associated with degenerating MNs in ventral horn both in SOD1G85R,or SOD1G93Amice (fast and slow strains). Spinal cord grey matter is delimited by a dotted line. Lumbar spinal cord sections from end-stage (postnatal (P) days in parentheses) mice overexpressing either human SOD1G85R (A-F) or SOD1G93A (G-L and M-R for the fast and slow strains , respectively) were double immunostained with AJ10 (red) and the neuronal marker NeuN (green); the large amount of AJ10-immunopositive profiles seen in (A, D, G, J and M, P), do not co-localize with apparently healthy NeuN-posi-tive neuronal cell bodies (B, C, E, F, H, I, K, L, N, O, Q, R). By contrast, AJ10 immunopositive neuronal cell bodies (encircled in J-L and P-R) no not display NeuN immunoreactivity. In (S-U) AJ10 immunoreactivity (red) was colocalized with ubiquitin (green). Some of the AJ10-positive mutant SOD1 aggregates (encircled in S-U) exhibit intense ubiquitin immunoreactivity; however, AJ10- positivity associated with neuronal processes (arrows in S-U), do not show ubiquitin immunoreactivity. Scale bar in C and F = 100 µm; U = 10 µm.

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