Lack of PCAF and p300 Involvement in ERCC1 Accumulation Induced by Cisplatin in H358 Cells

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This study explores the role of PCAF and p300 in ERCC1 accumulation following cisplatin exposure in H358 cells. Western blot analysis was conducted after transfection with control, p300 siRNA, or PCAF siRNA and subsequent cisplatin treatment.

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Jun 21, 2025

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Lack of PCAF and p300 Involvement in ERCC1 Accumulation Induced by Cisplatin in H358 Cells

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Supplementary Figure 4 A B Ctl sip300 Ctl siPCAF p300 PCAF ERCC1 ERCC1 Actin Actin Cisp (2h) Cisp (2h) PCAF and p300 are not involved in ERCC1 accumulation in response to cisplatin. H358 cells were transfected for 72 hours with either control (Ctl), p300 (A) or PCAF (B) siRNA and exposed to 50M cisplatin for an additional 2 hours. Western blotting was performed using anti-PCAF, anti-p300 and anti-ERCC1 antibodies. Actin was used as a loading control