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Supplementary Figure 2

HCT116. HCT116. NT. SN. NT. SN38. 5. 1.6. LC3 labelling. 4. 1.2. SN38 IC50 (nM). 3. 0.8. LC3 spots/cell. LC3. 0.4. 2. HCT116. Actin. 0. 1. Clonogenic assay. SN38. siCT. siATG5. siATG7. 0. shLUC. shMAPK14. HCT116. NT. SN38. 3. LC3. Cytotoxic assay. 100. GAPDH.

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Supplementary Figure 2

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  1. HCT116 HCT116 NT SN NT SN38 5 1.6 LC3 labelling 4 1.2 SN38 IC50 (nM) 3 0.8 LC3 spots/cell LC3 0.4 2 HCT116 Actin 0 1 Clonogenic assay SN38 siCT siATG5 siATG7 0 shLUC shMAPK14 HCT116 NT SN38 3 LC3 Cytotoxic assay 100 GAPDH 2 80 % living cells SN38 IC50 (nM) 60 1 40 20 0 0 siCT siAtg5 siAtg7 NT SN38 SN38+3MA HCT116 HCT116 Supplementary Figure 2 A B C D E F

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