Investigating the Role of IGFBP-5 in Tumor Growth and Blood Vessel Formation in Various Cell Lines
This study explores the expression and impact of IGFBP-5 mRNA in HEK293, MRC-5, SKOV-3, and MDAH-2774 cell lines. By assessing tumor volume (mm²) and number of blood vessels per tumor section, we evaluate the role of IGFBP-5 in tumor progression. Additionally, we analyze the effects of various concentrations of IGF-1 on IGFBP-5 expression, using anti-IGFBP-5 and anti-β-actin as controls. Supplementary Figures outline experimental data supporting our findings on VEGF interaction and tumor vascularization.
Investigating the Role of IGFBP-5 in Tumor Growth and Blood Vessel Formation in Various Cell Lines
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Presentation Transcript
A HEK293 MRC-5 SKOV-3 MDAH-2774 Igfbp5 mRNA Gapdh mRNA anti-IGFBP-5 anti-b-actin B mock IGFBP-5 mock IGFBP-5 2000 1500 anti-CD31 (PECAM-1) tumor volume (mm2) 1000 * 500 0 mock IGFBP-5 20 15 number of blood vessels per tumor section 10 * 5 0 mock IGFBP-5 Supplementary Figure 1 Supplementary Figure 2
VEGF untreated IGF-1 (100 ng/ml) Igfbp5 mRNA Gapdh mRNA anti-IGFBP-5 anti-b-actin untreated 10 ng/ml 50 ng/ml 100 ng/ml anti-IGFBP-5 anti-b-actin Supplementary Figure 3 Supplementary Figure 4
