1 / 36

Simplifying Laboratory Test Interpretation

Simplifying Laboratory Test Interpretation . Maria del Rosario, MD, MPH Division of Infectious Disease Epidemiology WVDHHR/BPH/OEPS May 2011. Objectives. Review laboratory tests commonly encountered in public health surveillance.

cirila
Télécharger la présentation

Simplifying Laboratory Test Interpretation

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Simplifying Laboratory Test Interpretation Maria del Rosario, MD, MPH Division of Infectious Disease Epidemiology WVDHHR/BPH/OEPS May 2011 WVDHHR/BPH/OEPS/DIDE

  2. Objectives • Review laboratory tests commonly encountered in public health surveillance. • Discuss laboratory test reports and practice report interpretation using specific examples. Disclaimer: This lecture is not intended to replace the advice and recommendations of a healthcare provider. WVDHHR/BPH/OEPS/DIDE

  3. Definition of Terms • Normally sterile site: sites in the human body that are normally free from organisms or foreign material, e.g. blood, joint, brain, etc. • Unsterile site: sites in the human body that generally harbor microorganisms, e.g. gut, oral cavity, nose, skin, etc • Specimen: a sample of tissue (blood, urine, etc.) that may or may not contain organisms • Isolate: a population of organisms (bacteria) that has been separated from a mixture • Serotype: a group of closely related organisms with distinct characteristics. • Assay: A test to detect or quantify a substance in a sample. WVDHHR/BPH/OEPS/DIDE

  4. Laboratory Tests • Detection Methods • Microscopy • Culture • Antigen test* • Identification Methods • PCR* • Viral load* • PFGE • Genotyping • Serology • Antimicrobial susceptibility • Ancillary tests *both detect and identify WVDHHR/BPH/OEPS/DIDE

  5. Microscopy Direct examination of a specimen (or may use stains) to detect the presence of organisms. Pros: • Quick and easy • Preliminary results Cons: • Not specific Gram negative diplococci WVDHHR/BPH/OEPS/DIDE

  6. Culture The process of growing and propagating organisms in a media that is conducive for their growth. S. pneumoniae on blood agar plate Pros: • Confirm the organism • Reproduce the organism and use for additional testing Cons: • Delay in confirmation • Require viable organism • Difficult for fastidious organisms colony WVDHHR/BPH/OEPS/DIDE

  7. Antigen Test Use of assay to detect the presence of antigen/s. Some assays are able to differentiate antigens, some are not able to. WVDHHR/BPH/OEPS/DIDE

  8. Result 1 • Purpose of test • Test result interpretation WVDHHR/BPH/OEPS/DIDE

  9. Polymerase Chain Reaction (PCR) Method used to amplify a specific region of a DNA strand. Pros: • Simple process, eliminates tedious work, results available within a day • Does not require a viable organism since only a strand of DNA is needed, • Sensitive test Cons: • Sensitive – pick up environmental contaminants • Unable to distinguish between certain species WVDHHR/BPH/OEPS/DIDE

  10. Result 2 • Purpose of test • Test result interpretation WVDHHR/BPH/OEPS/DIDE

  11. Pulsed Field Gel Electrophoresis (PFGE) The outbreak strain of SalmonellaTyphimurium has been found in ill humans and in food samples during this outbreak investigation. A technique to separate large DNA molecules by applying an electric field that periodically changes direction (electrophoresis)…to compare DNA banding patterns (fingerprints). WVDHHR/BPH/OEPS/DIDE

  12. Serology • Serology: the study of blood serum, with emphasis on testing of antibodies in the serum • Antigen: A substance which stimulates the body to produce antibody; usually a ‘foreign’ substance • Antibody: A protein molecule produced by the body’s immune system in response to a specific antigen. The antibody combines with the antigen and disables it. • Also called Immunoglobulins (e.g. IgG, IgM, IgA, IgE) • Referred to as anti-(name of antigen), e.g. anti-HCV, anti-HAV WVDHHR/BPH/OEPS/DIDE

  13. Antibodies • IgM: type of antibody produced by the body, usually the first antibody to appear in response to a foreign substance exposure, then eliminates the organism in the early stages of immunity before there is sufficient IgG • IgG: type of antibody that provides the majority of antibody-based immunity against invading organisms. The only antibody that crosses the placenta to provide immunity to the fetus • Titer: the amount of antibodies present in the blood, usually as a result of infection. • Acute titer and Convalescent titer: At the acute stage of disease, serum is tested (acute phase), followed by another blood draw and testing about 3 weeks (convalescent phase) later. IgG levels are compared and a 4-fold increase between acute and convalescent samples usually indicate infection. WVDHHR/BPH/OEPS/DIDE

  14. Basic Anatomy of Antibody Response to Infection WVDHHR/BPH/OEPS/DIDE

  15. Human Parvovirus B-19: Disease and Immune Response http://www.stanford.edu/group/virus/parvo/2005/B19.html WVDHHR/BPH/OEPS/DIDE

  16. Antibody Testing Pros: • Screening tool • Readily available • Indicates response to antigen (even if antigen is not detectable) – may indicate infection or immunity Cons: • Paired testing necessary for some diseases - may take a while to get results, impact on patient management • Unable to differentiate between immunity and disease • Sensitivity and specificity: • False-negative result: compromised immune system • False-positive result: liver disease, low disease prevalence WVDHHR/BPH/OEPS/DIDE

  17. Result 3 IFA • Type of test • Purpose of test • Test result interpretation WVDHHR/BPH/OEPS/DIDE

  18. Ehrlichiachaffeensis Infection Laboratory criteria for diagnosis Supportive: Serological evidence of elevated IgG or IgM antibody reactive with E. chaffeensis antigen by IFA, ELISA, dot-ELISA, or assays in other formats (CDC uses an IFA IgG cutoff of ≥1:64 and does not use IgM test results independently as diagnostic support criteria.), OR … Confirmed: Serological evidence of a fourfold change in immunoglobulin G (IgG)-specific antibody titer to E. chaffeensis antigen by IFA between paired serum samples (one taken in first week of illness and a second 2-4 weeks later), OR Detection of E. chaffeensis DNA …OR Demonstration of ehrlichial antigen…, OR Isolation of E. chaffeensis from a clinical specimen… WVDHHR/BPH/OEPS/DIDE

  19. Hepatitis A Antibody Tests • Hepatitis A antibody Total • Anti-HAV Total • Antibody to Hepatitis A Virus • HAV Ab Total • - measures both IgM and IgG • Hepatitis A antibody IgM • Anti-HAV, IgM • Antibody to Hepatitis A Virus, IgM • HAVAb, IgM WVDHHR/BPH/OEPS/DIDE

  20. Type of test • Purpose of test • Test result interpretation Result 4 WVDHHR/BPH/OEPS/DIDE

  21. HCV RNA HCV RNA HCV RNA HCV RNA WVDHHR/BPH/OEPS/DIDE

  22. Hepatitis C Testing - 1 SEROLOGIC TESTS • Enzyme Immunoassay (EIA) for Anti-HCV • Positive: past or current infection • Verification of Anti-HCV (+) screening test • Reflex supplemental testing*: follow-up with more specific serologic test, e.g. HCV RIBA or NAT • Signal-to-cut-off ratio (s/co): predict supplemental test-positive results ≥95% of the time, s/co dependent on test type • HCV RIBA* (Recombinant Immunoblot Assay) • Detects antibodies to individual HCV antigens and confers increased specificity compared to EIA-2 • Some RIBA-positive patients are HCV RNA-negative WVDHHR/BPH/OEPS/DIDE

  23. WVDHHR/BPH/OEPS/DIDE

  24. Hepatitis C Testing - 2 VIRAL LOAD TESTS • Measure HCV RNA (genetic material) • Detects actively replicating virus • 2 types: • Qualitative test - detects presence of HCV RNA virus (result: positive/negative) • Nucleic Acid Test (NAT)* for HCV RNA using RT-PCR • Detects HCV RNA in the blood • Very sensitive B. Quantitative test – measures the amount of virus in 1 ml of blood, use to assess response to treatment • Branched-chain DNA (bDNA) • Easy and cheap, especially for large number of samples • Only measures viral loads greater than 50 IU/ml • Transcription-mediated Amplification (TMA) • New, easy • Amplifies and detects viral genetic materia;l in the blood • Can measure viral loads as few as 5-10 IU/ml WVDHHR/BPH/OEPS/DIDE

  25. Hepatitis C Testing - 3 GENOTYPING • HCV Genotype • 6 genotypes, >50 subtypes • clinical importance: counseling and treatment • epidemiology LIVER FUNCTION TEST • ALT • SGPT WVDHHR/BPH/OEPS/DIDE

  26. WVDHHR/BPH/OEPS/DIDE

  27. Type of test • Purpose of test • Test result interpretation Result 5a WVDHHR/BPH/OEPS/DIDE

  28. Hepatitis C, past or present Clinical Case Definition • No symptoms are required… Laboratory criteria for diagnosis • 1 or more of following 4 criteria: Anti–HCV positive (repeatedly reactive) EIA verified by at least 1 additional more specific assay, OR • HCV RIBA positive, OR • NAT positive for HCV RNA (including genotype), OR • Anti-HCV screening-test-positive with a signal to cut-off ratio predictive of a true positive as determined for the particular assay and posted by CDC. Case classification • Confirmed: laboratory confirmed and does not meet the case definition for acute hepatitis C. • Probable: anti-HCV positive (repeat reactive) by EIA and has ALT or SGPT values above the upper limit of normal, but the anti-HCV EIA result has not been verified by an additional more specific assay or the signal to cut-off ratio is unknown. WVDHHR/BPH/OEPS/DIDE

  29. Result 5b • Type of test • Purpose of test • Interpretation of • Test 1 • Test 2 Test 1 Test 2 WVDHHR/BPH/OEPS/DIDE

  30. Antimicrobial Susceptibility MIC (minimum inhibitory concentration) • lowest concentration of antimicrobials that will inhibit the growth of organisms. MICs are important to confirm resistance of organisms to an antimicrobial agent. Methods: • Disk diffusion test • E test • Broth dilution test Zone of Inhibition MIC WVDHHR/BPH/OEPS/DIDE

  31. Sample 6 • Type of test • Purpose of test • Test result interpretation WVDHHR/BPH/OEPS/DIDE

  32. Sample 7 • Type of test • Purpose of test • Test result interpretation WVDHHR/BPH/OEPS/DIDE

  33. Ancillary Tests • CBC and WBC • CSF cells • Liver function tests – ALT, AST, bilirubin WVDHHR/BPH/OEPS/DIDE

  34. Tips when reviewing a laboratory report • Is the organism (or disease) reportable? • When was the specimen obtained in relation to onset of illness? • Was the source from a normally sterile site? • Were antibiotics used prior to specimen collection? WVDHHR/BPH/OEPS/DIDE

  35. Summary • Basic understanding of a laboratory test is key to maximizing its use. • Laboratory tests have ‘strengths’ and ‘weaknesses’. • Timing is everything! (between disease onset and specimen collection) WVDHHR/BPH/OEPS/DIDE

  36. Thank you Comments and Questions WVDHHR/BPH/OEPS/DIDE

More Related