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Enzyme Lab Write-Up Instructions and Toothpickase Demonstration

Follow instructions to complete enzyme lab write-up, understand enzyme factors, and watch Toothpickase demo. Explore enzyme reactions in different scenarios and learn about denaturation and protein shape. Use worksheet for further learning.

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Enzyme Lab Write-Up Instructions and Toothpickase Demonstration

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  1. Have your enzyme lab write up out on your table. • If you did not finish it you need to let me know ASAP. • Everyone that did finish should move to your table’s computer. • Your task is to type up the final lab by going through the answers and creating the best answer for each question. You are NOT just picking one persons answers. • You should put the most updated procedure in. This means if you edited the procedure by hand you should include these edits. • When you are done. Print it & staple everyone's enzyme write up to the back • You have 30 minutes or less. If you finish early work on something for another class.

  2. Factors that effect enzymes… • In your lab you tested the effect of different factors on enzymes. • To REALLY understand what is happening we will do a simple demonstration with toothpicks.

  3. Toothpickase Demo • 1 person in your group will break the toothpicks completely in ½ using only 3 fingers (cannot use the table as leverage) • You may not look at the table

  4. Toothpickase Demo • Enzyme • Substrate • Active site • Products • Transition state • Person • Toothpicks • 3 fingertips • Broken toothpicks • Moment of breaking

  5. Situation #1 – Low Concentration • Spread 25 toothpicks around the table. Use the full area of the table • “enzyme” person (with eyes closed) has 15s to break as many toothpicks as possible toothpicks

  6. Situation #2 – High Concentration • Concentrate 25 toothpicks in front of the person who will play the enzyme. • Only break one toothpick at a time! • You have 15s to break toothpicks

  7. Situation #3 – Cold Temperatures • This person should have held their hand in the ice bath for about 4-5 minutes • Concentrate 25 toothpicks in front of their seat • They have 15s to break toothpicks

  8. Situation #4 - Denaturing • Excessive heat, acid/base, salt concentration disrupts the bonding of the enzyme, breaking it apart. The protein looses it’s normal shape.

  9. Situation #4 - Denatured • Cross your middle and index fingers. Try to break the toothpick (you may not use the table to break it) • You have 15s

  10. What exactly IS denaturing?

  11. Remember: Enzyme activity depends on shape

  12. Denaturation • Thus a change in shape (denaturing) means a non-functional active site and no catalysis.

  13. Protein Shape

  14. Formed by Weak Bonds • Held together by weak bonds (ionic bonds, hydrogen bonds, hydrophobic interactions)

  15. How Acid/Base/Salts break those bonds

  16. Denaturation

  17. Enzymes Worksheet • Complete from knowledge/memory first in one color • Then fill in with notes/questions/help • I recommend pencil • http://www.youtube.com/watch?v=NdMVRL4oaUo enzyme song • Finish for HW

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