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Tubular Organs

Tubular Organs. Tracheal Development. DB. DT. LT. Dysfusion (Dys) is a bHLH-PAS Protein. bHLH. PAS-1. PAS-2. DB. DT. LT. Btl-GAL4, UAS-lacZ Dys. Dysfusion Is Expressed In Fusion Cells. dys Mutants. dys 3. dys 2. bHLH. PAS-1. PAS-2. WT. dys. Mab 2A12.

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Tubular Organs

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  1. Tubular Organs

  2. Tracheal Development DB DT LT

  3. Dysfusion (Dys) is a bHLH-PAS Protein bHLH PAS-1 PAS-2

  4. DB DT LT Btl-GAL4, UAS-lacZ Dys Dysfusion Is Expressed In Fusion Cells

  5. dys Mutants dys 3 dys 2 bHLH PAS-1 PAS-2 WT dys Mab 2A12

  6. How dys expression is regulated? How Dys:Tgo regulates down-stream target genes?

  7. Dysfusionis Expressed in Mutiple Cell Types

  8. Identify dys Enhancer

  9. Identify dys Fusion Cell Enhancer

  10. Ongoing…… • How dys expression is regulated? • Is dys auto-regulated by Dys:Tgo? • N283-GFP,dys/Tm3, twi-gal4, UAS-GFP • C1.7-GFP, dys/TM3, twi-gal4, UAS-GFP • Is dys regulated by esg? • esg/cyo, twi-gal4, UAS-GFP; N283 • esg/cyo, twi-gal4, UAS-GFP; C1.7 • Identify other transcription factors binding sites in dys enhancer. • Identify dys fusion cells enhancer in C1.7 and G1.7

  11. Summary of dys Function Dys Tgo Dys Dys Tgo Tgo N Identified dys Targets CG13196, E-cadherin (cell adhesion) TCGTG CG15252 (unknown function) members only (Protein trafficking) Trachealess (Migration)

  12. Two TCGTG Sites are Required to Activates CG13196

  13. Two TCGTG Sites of CG13196 are Conserved

  14. Two TCGTG Sites are Required to Activates CG15252

  15. Two TCGTG Sites of CG15252 are Conserved

  16. Potential Co-activator of Dys:Tgo is Required to Turn on Gene Expression in Additional Fusion Cells

  17. Ongoing..

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