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Donor ABO Blood Grouping Discrepancies

Donor ABO Blood Grouping Discrepancies. Heather Hume Greg Denomme CBS HO Serology Laboratory Feb 20, 2009. Donor ABO Blood Grouping Discrepancies. This part of the presentation shows the initial results only.

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Donor ABO Blood Grouping Discrepancies

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  1. Donor ABO Blood Grouping Discrepancies Heather Hume Greg Denomme CBS HO Serology Laboratory Feb 20, 2009

  2. Donor ABO Blood Grouping Discrepancies • This part of the presentation shows the initial results only. • Look at these results and think about the next steps in the investigation of the ABO grouping discrepancies. • Investigations will be discussed on the teleconference. • Presentation with the investigation will be made available just before the teleconference.

  3. References • Westhoff CM, Reid ME. ABO and related antigens and antibodies. In Blood Banking and Transfusion Medicine Basic Principles and Practice 2nd ed. Hillyer CD et al eds. Churchill Livingstone, Philadelphia, PA. 2007: 69-79. • ABO, Lewis and P groups and Ii antigens. In Mollison's Blood Transfusion in Clinical Medicine 11th ed. Klein HG, Anstee DJ eds. Blackwell Publishing Inc, Malden, MA. 2005:114-162. • Investigating ABO typing problems. In Judd's Methods in Immunohematology 3rd ed. Judd WJ, Johnson SJ, Storry JR eds. AABB Press, Bethesda, MA. 2008: 515-558

  4. GAL GAL GLNAC GLNAC GAL GAL GL GL GALNAC GAL GAL GLNAC GAL GL Group ‘O’ Antigen Group ‘B’ Antigen Group ‘A’ Antigen

  5. Basic ABO Grouping

  6. ABO Blood Grouping Discrepancies • Missing reactivity? • apparent absent antigen • apparent absent antibody • Additional reactivity? • apparent extra antigen • apparent extra antibody

  7. ABO Blood Grouping Discrepancies Most Common Causes • Patients • decreased antibody levels • physiologic - newborns, elderly • immunodeficiency - congenital or acquired • auto- or alloantibodies • recent transfusion • HSCT • Donors • unusual blood group - genetic polymorphisms

  8. Donor Testing Laboratory Results Case #1 PK7200 Data Log Results Prediluted Olympus reagents used DTL Manual Bench Results Immucor Gamma-clone reagents used

  9. Donor Testing Laboratory Results Case #2 PK7200 Data Log Results Prediluted Olympus reagents used DTL Manual Bench Results Immucor Gamma-clone reagents used

  10. Donor Testing Laboratory Results Case #3 PK7200 Data Log Results Prediluted Olympus reagents used DTL Manual Bench Results Immucor Gamma-clone reagents used MF=mixed field

  11. Donor Testing Laboratory Results Case #4Unit returned to CBS - Labelled B pos, tested AB pos at hospital PK7200 Data Log Results Prediluted Olympus reagents used DTL Manual Bench Results Immucor Gamma-clone reagents used MF=mixed field

  12. Donor Testing Laboratory Results Case #5 PK7200 Data Log Results Prediluted Olympus reagents used DTL Manual Bench Results Immucor Gamma-clone reagents used

  13. Donor Testing Laboratory Results Case #6 PK7200 Data Log Results Prediluted Olympus reagents used DTL Manual Bench Results Immucor Gamma-clone reagents used

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