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Iron-Reducing Bacteria

This presentation from the Hopkins Microbiology Course by Ying Lei on July 22, 2010, focuses on iron-reducing bacteria and their role in biogeochemical processes. It highlights the reduction of ferric iron (Fe3+) to ferrous iron (Fe2+) and the significance of magnetite formation. The presentation details an experimental setup for bacterial enrichment in anoxic conditions using Pfennig bottles, and discusses the implications for aquatic geomicrobiology. This content is crucial for understanding microbial interactions with iron minerals and their environmental impact.

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Iron-Reducing Bacteria

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  1. Iron-Reducing Bacteria Hopkins Microbiology Course Presented by Ying Lei 22 July 2010

  2. Reduction Process • Fe3+ only– ferrihydrite (FeOOH · 0.4H2O)– orange/red solid • Fe3+/Fe2+– magnetite (Fe3O4)– black, ferromagnetic solid • Fe2+ only– ferrous iron– black, non-magnetic solid

  3. Redox Tower CH3CH(OH)COO- + 12 FeOOH + 4 H2O  3 HCO3- + 12 Fe2+ + 22 OH- lactate C0 iron (III) Fe3+ CO2 C4+ iron (II) Fe2+ Canfield et al, Aquatic Geomicrobiology (2005)

  4. Enrichment Medium Adjust to pH 7.2 in Pfennig bottles and incubate under anoxic conditions. Hopkins Microbiology Course (2010)

  5. Sample Collection Canfield et al, Aquatic Geomicrobiology (2005)

  6. Sample Collection

  7. Successful Enrichments Marcus

  8. Thank You All Instructors and Students Chris Marcus Blanca

  9. Additional Slides

  10. Iron Solubility OzCoasts (www.ozcoasts.org.au)

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