1 / 24

BOLD Contrast: Functional Imaging with MRI

BOLD Contrast: Functional Imaging with MRI. Mark A. Elliott, PhD Department of Radiology University of Pennsylvania. Overview. Mechanisms of functional imaging with MRI Methodology of fMRI Issues for animal studies Spatial and temporal sensitivy of fMRI. electrical activity. - excitatory.

draco
Télécharger la présentation

BOLD Contrast: Functional Imaging with MRI

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. BOLD Contrast:Functional Imaging with MRI Mark A. Elliott, PhD Department of Radiology University of Pennsylvania

  2. Overview • Mechanisms of functional imaging with MRI • Methodology of fMRI • Issues for animal studies • Spatial and temporal sensitivy of fMRI

  3. electrical activity - excitatory - inhibitory - soma action potential electrophysiology Methods for Imaging Neural Activity metabolic response FDG PET - ATP tightly regulated - glucose consumption - oxygen consumption H215O PET hemodynamic response - blood flow fNIR - blood volume - blood oxygenation fMRI EEG MEG Perfusion MRI

  4. Vascular Sensitivity offMRI and fNIR Arterial Venous II I fNIR Intravascular Perfusion MRI II IV fMRI III I Extravascular III IV Vessel Size

  5. Vascular Response fMRI vs fNIR

  6. Mechanisms of fMRI Signal:BOLD Contrast Neural Activity “Flooding the garden to feed the thirsty flower” - ??? CMR02 CBF BOLD ( CBF - CMR02) spatial dimension • Hemodynamic response is a surrogate marker for neural activity • BOLD = Blood Oxygenation Level-Dependent • BOLD signal is a complex interaction of CBF + CBV + CMRO2: • CBF >> CMRO2 less deoxyhemoglobin with activation • CBF is monitored indirectly • “Tracer” is primarily venous • “Tracer” is endogenous

  7. Magnetic Susceptibility Affects Background Magnetic Field : permeability r : relative permeability M: magnetic susceptibility For biological tissues, | M | << 1 Diagmagnetic: M < 0 Paramagnetic: M > 0 The interface between regions with different M behaves like a magnetized dipole, perturbing the local B field.  M creates larger B B1  B2 B1 M1 B2 M2

  8. BOLD Contrast: Changes in Magnetic Susceptibility of Blood • Blood and brain tissue are diamagnetic. • Hb0 is diamagnetic. • Hb is strongly paramagnetic. • HbO is paramagnetic. • Increased Neuronal activity: • blood flow increases ≈ 30% • 02 consumption increases ≈ 5% • [Hb0]  • [Hb]  • Decrease in [Hb] reduces the M between blood and brain tissue • Magnetic field becomes more uniform  MRI signal affected

  9. Hypoxia Normoxia Hemoglobin Saturation AffectsMagnetic Field Homogeniety Rat brain, 7T from Ogawa, 1990 Field Map vs. Hemoglobin Saturation from Bandettini and Wong, 1995

  10. Summary: BOLD Contrast in fMRI Verbal Fluency Task Broca’s area Wernicke’s area • BOLD = Blood Oxygenation Level-Dependent • Oversupply of CBF raises [HbO] in regions of increased CMRO2 • Susceptibility mismatch between blood and tissue is reduced • Magnetic field becomes more homogeneous • Temporal T2* contrast generated in T2* sensitive MRI

  11. fMRI Methodology: Acqusition structural T1 weighted ~ 5 min Temporal series of EPIs . . . . 1x1x1 mm voxels EPI functional T2* weighted ~ 2 sec/volume ~ 300 images time ~ 10 min 3x3x3 mm voxels

  12. fMRI Methodology: Stimulus Blocked Design, Event-Related Design, and ISI Fixed ISI ISI On Blocked Design Off On Event Related Off Event related designs can have either fixed or variableinter-stimulus interval (ISI) ISI Variable ISI • Variable ISI allows for more stimuli per time. • Increased statistical power in analysis.

  13. fMRI Methodology: Analysis “Non-Activation” Signal Stimulus . . . . Processing “Activation” Signal Processing Stimulus

  14. T2*-weighted Snapshot Image Average Difference Image Statistical Significance Image Thresholded Statistical Image Overlay on Anatomic Image Brain Activation MapsStatistical Parametric Mapping ON task OFF signal courtesy J. Detre

  15. Hemodynamic Response Function The “HRF” - the theoretical impulse response of BOLD contrast to brief neuronal activity Peak Contrast Amplitude FWHM Onset Time Stimulus Time to Peak

  16. fMRI Model: HRF Linear System Linear Model Assumption y = hx Expected signal (y) is convolution of the stimulus signal (x) with the HRF (h) stimulus (x) HRF (h) signal (y) Signal is predicted for any arbitrary sequence of stimuli

  17. Applications of fMRI • Cognitive Neuroscience • Localization of sensorimotor and cognitive function • Brain-behavior correlations • Clinical Neuroscience • Presurgical mapping • Differential diagnosis of cognitive disorders • Recovery of function/neuroplasticity Photic Stimulation

  18. Implications for Animal fMRI • Pharmacological effects on neuronal metabolism and hemodynamic response • Small voxel sizes reduce SNR • Smaller volumes enable higher field magnets (7 and 9.4T) • Passive stimulus delivery (training possible in some models)

  19. T2* Signal Loss in the Pre-Frontal Cortex Air is highly paramagnetic (like Hb) Air-tissue interface has “static” M Background signal “drop-out” F B0 E 1 S B0 2 Bn = Normal component Bn = Tangential component F = frontal sinus E = ethmoidal sinus S = sphenoidal sinus Normal component is unchanged by  B1n = B2n Tangential component is altered by B1n = 1 / 2 B2n

  20. Signal Dropout in T2* Weighted Images Increasing TE

  21. Spatial Extent of BOLD Neural Activity CMR02 Hb Saturation (%, approx.) restingactive arterioles 90 90 capillaries 80 90 veins 60 90 CBF BOLD ( CBF - CMR02) microvessels draining veins Positive T2* contrast derived from CBF > CMR02 Venous compartment experiences largest Hb (and T2*) Draining veins are less spatially specific to site of neural activity

  22. Extravascular BOLD Signal B0 “inhomogeneity” from vessel extends into extravascular (EV) space EV Magnetic Field Gradient microvessel macrovessel • Diffusion of water molecules through B0 gradients • Large vessels: static dephasing, T2* effect • Small vessels: dynamic dephasing, T2 and T2* effect • Spin-echo fMRI less sensitive to large vessel (venous) • extravascular space water diffusion from Principles of Functional MRI, Seong-Gi Kim

  23. Echo Time and Field Strength Effects on BOLD Contrast • BOLD contrast increase with echo time (TE) • SNR decreases with echo time • Optimal CNR when TE  resting T2* • BOLD contrast increases with magnetic field • SNR increases with magnetic field 1.5T % Signal TE (msec) 3T % Signal TE (msec) from Stroman et al, Proc. ISMRM, Glasgow (2001)

  24. Field Strength Effecton BOLD Spatial Sensitivity • T2* of blood shortens quadratically with B0 • Field dependence of T2,blood T2,tissue • - Decreased venous contribution Diffusion weighted BOLD Intravascular BOLD component model simulation Rat brain, 9.4T from S.P. Lee et al, (2003)

More Related