1 / 11

Ex. 17: Nitrate Respiration (Nitrate Reduction Test)

Ex. 17: Nitrate Respiration (Nitrate Reduction Test). Objectives?. Vocabulary. Oxidation – reduction reactions Reductase Nitrate vs. Nitrite Denitrification. . Some bacteria only produce nitr a te reductase, other bacteria also produce nitr i te reductase.

elias
Télécharger la présentation

Ex. 17: Nitrate Respiration (Nitrate Reduction Test)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Ex. 17: Nitrate Respiration (Nitrate Reduction Test) Objectives?

  2. Vocabulary • Oxidation – reduction reactions • Reductase • Nitrate vs. Nitrite • Denitrification

  3. Some bacteria only produce nitrate reductase, other bacteria also produce nitrite reductase Nitrate Reduction and Denitrification nitrite reductase nitrate reductase

  4. Day 1:

  5. Reagents and Steps • Test for accumulation of nitrite: Add 5 drops of nitrate reagents A (dimethyl-naphthylamine) and B (sulfanilic acid)  red color means nitrite present (+) • If step one is negative: Add small pinch of Zinc powder  red means nitrate was present. Why? And what is the meaning? • No color development in step 1 or 2  Why? And what is the meaning?

  6. Possible Results and Interpretation

  7. Nitrate Reduction Test Negative Control P. aeru- ginosa Test tubes after 24 hour incubation and before adding reagents A and B A. faecalis P. vulgaris

  8. . . . after adding reagents A and B Negative Control P. aeru- ginosa A. faecalis P. vulgaris What do you do next?

  9. . . . after adding Zinc to tubes 1, 2 and 4 P. aeru- ginosa Negative Control A. faecalis P. vulgaris

  10. Step 1 Step 2

  11. Step 1 ??? Step 2 the end

More Related