PCR Amplification of Genes in A. tumefaciens Transconjugants from pSymA and pSymB Plasmids

Jan 31, 2026

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This study investigates the PCR amplification of genes from the pSymA and pSymB plasmids in Agrobacterium tumefaciens GMI9023 transconjugants. The recipient strain, AtGMI9023, exhibited PCR-negative results for all marker genes, while the donor strain 1021RctAHB served as a positive control. Transconjugants 1 and 2 amplified only the SMa1077 and SMb20094 genes, whereas other transconjugants showed positive amplification for all five genes tested. The M100bp DNA molecular weight marker was utilized for reference.

elijah-erickson

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PCR Amplification of Genes in A. tumefaciens Transconjugants from pSymA and pSymB Plasmids

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Transconjugants (pSymA + pSymB) 100 bp 1021RctAHB AtGMI9023 200 bp M100bp M100bp 1 2 3 4 5 6 7 8 SMb21314 (wgeA) SMb20946 (exoY) SMb20094 SMa1077 (nex18) SMa4202 (rhbB) Figure S2. PCR amplication of genes from pSymA and pSymB in A. tumefaciens GMI9023 transconjugants that received both plasmids simultaneously. The recipient strain AtGMI9023 was PCR-negative for all marker genes, and the donor strain 1021RctAHB was used as a positive control. Note that transconjugants 1 and 2 only amplified SMa1077 and SMb20094 genes, whereas the remaining transconjugants examined gave positive amplification of all 5 genes tested. M100bp, DNA molecular weight marker.