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Transconjugants (pSymA + pSymB). 100 bp. 1021RctAHB. AtGMI9023. 200 bp. M100bp. M100bp. 1. 2. 3. 4. 5. 6. 7. 8. SMb21314 (wgeA). SMb20946 (exoY). SMb20094. SMa1077 (nex18). SMa4202 ( rhbB).
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Transconjugants (pSymA + pSymB) 100 bp 1021RctAHB AtGMI9023 200 bp M100bp M100bp 1 2 3 4 5 6 7 8 SMb21314 (wgeA) SMb20946 (exoY) SMb20094 SMa1077 (nex18) SMa4202 (rhbB) Figure S2. PCR amplication of genes from pSymA and pSymB in A. tumefaciens GMI9023 transconjugants that received both plasmids simultaneously. The recipient strain AtGMI9023 was PCR-negative for all marker genes, and the donor strain 1021RctAHB was used as a positive control. Note that transconjugants 1 and 2 only amplified SMa1077 and SMb20094 genes, whereas the remaining transconjugants examined gave positive amplification of all 5 genes tested. M100bp, DNA molecular weight marker.