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Fabrication of an electrospun nanofibrous scaffold for use in the field of tissue engineering

Fabrication of an electrospun nanofibrous scaffold for use in the field of tissue engineering. By: Shannon Daily & Tyler Crawford. Purpose.

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Fabrication of an electrospun nanofibrous scaffold for use in the field of tissue engineering

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  1. Fabrication of an electrospunnanofibrous scaffold for use in the field of tissue engineering By: Shannon Daily & Tyler Crawford

  2. Purpose • To create a polycaprolactone mesh which enables cell activity and seeks to eventually provide an application in the field of tissue engineering toward biomimetic skin graft.

  3. Progress since last meeting • Spun 4 more meshes • Chitosan included • Progress on cell work • Established cell line • Grew cells in tissue culture plates • Final Paper started • Materials and Method section

  4. Chitosan Solution • 1 wt.% Chitosan – Spun 3 meshes successfully • 2 wt.% Chitosan – Spun 1 mesh successfully • Plan to also spin 0.5 wt.% chitosan

  5. Mesh Chart *SEM images Cell work

  6. Mesh Chart continued

  7. Cell Work • Worked with Gabi and Allison to establish cell line • Seeded mesh #4 with cells in tissue culture plate • Seeded mesh #6 with cells • Left over 2 weeks – fibroblast cells observed growing in plate

  8. Cell Work - Problems • Contamination (Fungus and Bacteria) • Observed in Mesh #4 – 1st trial with cells with mesh • Possible solutions • increase sterilization time under UV light • Two trials of mesh in media with and without cells • Cells grew around the mesh in #6

  9. Additional Problems • SEM issues • Unable to use the one at Janelia Farm • No contact from other university • Mesh too thick to see through on microscope • Possibly make thinner meshes to be able to see cells

  10. To be worked on: • SEM images for meshes created • Cell work • Continue using different meshes (possibly try well plates) • Continuespinning PCL/chitosan solutions • Continue work for final symposium

  11. Bibliography Akhyari, P., Kamiya, H., Haverich, A., Karck, M., & Lichtenberg, A. (2008). Myocardial tissue engineering: The extracellular matrix. European Journal of Cardio-Thoracic Surgery, 34, 229-241. doi: 10.1016/j.ejcts.2008.03.062 Bhardwaj, N. & Kundu, S. C. (2010). Electrospinning: A fascinating fiber fabrication technique. Biotechnology Advances, 28, 325-347. doi: 10.1016/j.biotechadv.2010.01.004 Chong, E.J., Phan, T.T., Lim, I.J., Zhang, Y.Z., Bay, B.H., Ramakrishna, S., & Lim, C.T. (2007). Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution. ActaBiomaterialia, 3, 321-330. doi: 10.1016/j.actbio.2007.01.002 Geng, X., Kwon, O-H., & Jang, J. (2005). Electrospinning of chitosan dissolved in concentrated acetic acid solution. Biomaterials, 26, 5427-5432. Han, J., Branford-White, C.J., & Zhu, L.M. (2010). Preparation of poly(є-caprolactone)/poly(trimethylene carbonate) blend nanofibers by electrospinning. Carbohydrate Polymers, 79, 214-218. doi: 10.1016/j.carbpol.2009.07.052 Homayoni, H., Ravandi, S.A.H., & Valizadeh, M. (2009). Electrospinning of chitosannanofibers: Processing optimization. Carbohydrate Polymers, 77, 656-661. Lowery, J.L., Datta, N., & Rutledge, G.C. (2010). Effect of fiber diameter, pore size and seeding method on growth of human dermal fibroblasts in electrospun poly(є-caprolactone) fibrous mats. Biomaterials, 31, 491-504. doi: 10.1016/j.biomaterials.2009.09.072 Nisbet, D.R., Forsythe, J.S., Shen, W., Finkelstein, D.I., & Horne, M.K. (2009). A review of the cellular response on electrospun nanofibers for tissue engineering. Journal of Biomaterials Application, 24, 7-29. Pham, Q.P., Sharama, V., & Mikos, A.G. (2006). Electrospinning of polymeric nanofibers for tissue engineering applications: A review. Tissue Engineering, 12,1197-1211. Shevchenko, R.V., James, S.L., & James, S.E. (2010). A review of tissue-engineered skin bioconstructs available for skin reconstruction. Journal of the Royal Society Interface, 7, 229-258. doi: 10.1098/rsif.2009.0403 Sill, T.J., & von Recum, H.A. (2008). Electrospinning: Applications in drug delivery and tissue engineering. Biomaterials, 29, 1989-2006. doi: 10.1016/j.biomaterials.2008.01.011 Woodruff, M.A., & Hutmacher, D.W. (in press). The return of a forgotten polymer- Polycaprolactone in the 21st century. Progress in Polymer Science. doi: 10.1016/j.progpolymsci.2010.04.002

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