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3. Give a very general outline of the treatment of measured saxs data prior to interpretation.

1. a. Al alloys containing about 5 vol.% of Al 3 ZrTi precipitates (density = 2.92 gm/cc) were prepared by melt spinning (pouring a thin stream of the melt on a rapidly spinning copper wheel). The resulting melt-spun ribbons ranged in thickness from 15 to 40  .

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3. Give a very general outline of the treatment of measured saxs data prior to interpretation.

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  1. 1. a. Al alloys containing about 5 vol.% of Al3ZrTi precipitates (density = 2.92 gm/cc) were prepared by melt spinning (pouring a thin stream of the melt on a rapidly spinning copper wheel). The resulting melt-spun ribbons ranged in thickness from 15 to 40 . Describe how specimens of this material would be prepared for saxs measurements of the size of the precipitates. (CuK)

  2. 1. a. Al alloys containing about 5 vol.% of Al3ZrTi precipitates (density = 2.92 gm/cc) were prepared by melt spinning (pouring a thin stream of the melt on a rapidly spinning copper wheel). The resulting melt-spun ribbons ranged in thickness from 15 to 40 . Describe how specimens of this material would be prepared for saxs measurements of the size of the precipitates. (CuK) What are possible forms for specimens

  3. 1. a. Al alloys containing about 5 vol.% of Al3ZrTi precipitates (density = 2.92 gm/cc) were prepared by melt spinning (pouring a thin stream of the melt on a rapidly spinning copper wheel). The resulting melt-spun ribbons ranged in thickness from 15 to 40 . Describe how specimens of this material would be prepared for saxs measurements of the size of the precipitates. (CuK) What are possible forms for specimens Then, need to calc approx. thickness

  4. 1. a. Al alloys containing about 5 vol.% of Al3ZrTi precipitates (density = 2.92 gm/cc) were prepared by melt spinning (pouring a thin stream of the melt on a rapidly spinning copper wheel). The resulting melt-spun ribbons ranged in thickness from 15 to 40 . Describe how specimens of this material would be prepared for saxs measurements of the size of the precipitates. (CuK) What are possible forms for specimens Then, need to calc approx. thickness 1/= 1/48.6 x 2.92 = 0.0071 cm = 71 microns

  5. b. Describe the preparation of an E. coli asparaginase specimen for saxs study of the macromolecular shape in solution…different than the structural shape in crystalline form (shown below). The asparaginase is supplied as a very dilute suspension in a special buffer solution. (CuK)

  6. b. Describe the preparation of an E. coli asparaginase specimen for saxs study of the macromolecular shape in solution…different than the structural shape in crystalline form (shown below). The asparaginase is supplied as a very dilute suspension in a special buffer solution. (CuK) What type will the specimen be?

  7. b. Describe the preparation of an E. coli asparaginase specimen for saxs study of the macromolecular shape in solution…different than the structural shape in crystalline form (shown below). The asparaginase is supplied as a very dilute suspension in a special buffer solution. (CuK) What type will the specimen be? What window mat'l?

  8. b. Describe the preparation of an E. coli asparaginase specimen for saxs study of the macromolecular shape in solution…different than the structural shape in crystalline form (shown below). The asparaginase is supplied as a very dilute suspension in a special buffer solution. (CuK) What type will the specimen be? What window mat'l? Quartz?

  9. b. Describe the preparation of an E. coli asparaginase specimen for saxs study of the macromolecular shape in solution…different than the structural shape in crystalline form (shown below). The asparaginase is supplied as a very dilute suspension in a special buffer solution. (CuK) What type will the specimen be? What window mat'l? Quartz? = 34.43 1/* = 1/ 34.43 x 2.65 = 0.011 cm this is for a good scatterer…so should be much thinner (2 windows)

  10. b. Describe the preparation of an E. coli asparaginase specimen for saxs study of the macromolecular shape in solution…different than the structural shape in crystalline form (shown below). The asparaginase is supplied as a very dilute suspension in a special buffer solution. (CuK) What type will the specimen be? What window mat'l? Quartz? = 34.43 1/* = 1/ 34.43 x 2.65 = 0.011 cm this is for a good scatterer…so should be much thinner (2 windows) Polymer?

  11. b. Describe the preparation of an E. coli asparaginase specimen for saxs study of the macromolecular shape in solution…different than the structural shape in crystalline form (shown below). The asparaginase is supplied as a very dilute suspension in a special buffer solution. (CuK) What type will the specimen be? What window mat'l? Quartz? = 34.43 1/* = 1/ 34.43 x 2.65 = 0.011 cm this is for a good scatterer…so should be much thinner (2 windows) Polymer? (C only)= 4.60 1/* = 1/ 4.60 x 1 = 0.217 cm much better

  12. b. Describe the preparation of an E. coli asparaginase specimen for saxs study of the macromolecular shape in solution…different than the structural shape in crystalline form (shown below). The asparaginase is supplied as a very dilute suspension in a special buffer solution. (CuK) Convert suspension to solution Should be dilute

  13. 2. Describe the Kratky instrument in detail, showing, in particular, how the collimation is effected, and also where the specimen is placed. This instrument requires careful data treatment; why?

  14. 2. Describe the Kratky instrument in detail, showing, in particular, how the collimation is effected, and also where the specimen is placed. This instrument requires careful data treatment; why? Needs slit-desmearing

  15. 3. Give a very general outline of the treatment of measured saxs data prior to interpretation.

  16. 3. Give a very general outline of the treatment of measured saxs data prior to interpretation. Need: specimen in container data

  17. 3. Give a very general outline of the treatment of measured saxs data prior to interpretation. Need: specimen in container data empty container data std data

  18. 3. Give a very general outline of the treatment of measured saxs data prior to interpretation. Need: specimen in container data empty container data std data dark count detector efficiency bkgrd data incident beam intensity transmissions and thicknesses

  19. 3. Give a very general outline of the treatment of measured saxs data prior to interpretation. Need: specimen in container data empty container data std data dark count detector efficiency bkgrd data incident beam intensity 1scale all with this transmissions and thicknesses

  20. 3. Give a very general outline of the treatment of measured saxs data prior to interpretation. Need: specimen in container data empty container data std data dark count detector efficiency 2correct all bkgrd data incident beam intensity 1scale all with this transmissions and thicknesses

  21. 3. Give a very general outline of the treatment of measured saxs data prior to interpretation. Need: specimen in container data empty container data std data dark count 3subtract detector efficiency 2correct all bkgrd data incident beam intensity 1scale all with this transmissions and thicknesses

  22. 3. Give a very general outline of the treatment of measured saxs data prior to interpretation. Need: specimen in container data empty container data 4subtract using T/T ratios std data dark count 3subtract detector efficiency 2correct all bkgrd data incident beam intensity 1scale all with this transmissions and thicknesses

  23. 3. Give a very general outline of the treatment of measured saxs data prior to interpretation. Need: specimen in container data empty container data 4subtract using T/T ratios std data dark count 3subtract detector efficiency 2correct all bkgrd data incident beam intensity 1scale all with this transmissions and thicknesses corrected specimen data corrected bkgrd data corrected std data

  24. 3. Give a very general outline of the treatment of measured saxs data prior to interpretation. Need: specimen in container data empty container data 4subtract using T/T ratios std data dark count 3subtract detector efficiency 2correct all bkgrd data incident beam intensity 1scale all with this transmissions and thicknesses corrected specimen data corrected bkgrd data 4get I(q) s corrected std data I/Istdf(q)(tstdTstd/tT)

  25. 3. Give a very general outline of the treatment of measured saxs data prior to interpretation. Need: specimen in container data empty container data 4subtract using T/T ratios std data dark count 3subtract detector efficiency 2correct all bkgrd data incident beam intensity 1scale all with this transmissions and thicknesses corrected specimen data corrected bkgrd data 4get I(q) s corrected std data I/Istdf(q)(tstdTstd/tT) 5subtract bkgrd

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