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Molecular Signals to Synaptic Change: Understanding Long-Lasting Effects

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  1. The Time Machine: Transforming Molecular Signals into Long-Lasting Synaptic Change David Santucci Quantitative Biology Bootcamp 2009

  2. Presynaptic Release Long Term Potentiation Difference in time scale > 6 orders of magnitude

  3. Induction Expression Early Late

  4. LTP Induction Protocols

  5. Blocking NMDA Receptors Blocks LTP

  6. Uncaging Ca2+ Alone Can Induce LTP

  7. Long Term Depression

  8. Prot ProtK P P Prot Protein Kinases Catalyze the Phosphorylation of Proteins

  9. P P CaMKII

  10. Induction Expression Early Late

  11. LTP Leads to an Increase in Surface AMPA Receptors

  12. LTP Leads to an Increase in Spine Size

  13. Induction Expression Early Late

  14. Blocking Protein Synthesis Blocks the Late Phase of LTP

  15. LTP TAG Prot

  16. Weak Stimulus Followed by a Strong Stimulus Produces LTP

  17. Molecular Makeup of the PSD

  18. Scaffolding Molecules

  19. PSD Proteins Are In Flux

  20. Actin Treadmilling, Capping, Cutting and Cross-Linking

  21. Actin Treadmilling, Capping, Cutting and Cross-Linking

  22. QDOT AB AMPA Single-Particle Tracking of AMPA Receptor Diffusion

  23. Imaging of Isolated PSDs with Immunogold EM

  24. EM Tomography of Molecular-Scale Structures in the PSD

  25. Förster Resonance Energy Transfer (FRET)

  26. 488 nm 507 nm 405 nm 555 nm 572 nm Bleached Dendra2 Super-Resolution Imaging Using FPALM

  27. 488 nm 507 nm 405 nm 555 nm 572 nm Super-Resolution Imaging Using FPALM

  28. Super-Resolution Imaging Using FPALM

  29. Thanks to QB Program Jané Kondev Jeff Gelles Trisha Murray John Lisman Sri Raghavachari All of you...

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