1 / 1

Investigating Clonogenic Efficiency and Colony Formation in RKO Cells with FAK Knockdown

This study explores the impact of Focal Adhesion Kinase (FAK) knockdown on the clonogenic efficiency of RKO cells. By utilizing siRNA targeting FAK, we assess changes in colony formation through both adherent and suspension conditions. Key metrics include colony-forming frequency, normalized colony formation rates, and protein expressions like Paxillin phosphorylated at Y118 and GAPDH. The results aim to elucidate the role of FAK in cancer cell proliferation and adhesion, contributing valuable insights into therapeutic strategies.

gerik
Télécharger la présentation

Investigating Clonogenic Efficiency and Colony Formation in RKO Cells with FAK Knockdown

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. si GFP si GFP si GFP si FAK si FAK si FAK 60 100 50 80 40 60 Clonogenic efficiency (%) 30 40 20 20 10 0 HT-25 RKO 0 RKO HT-25 C HT-25 RKO A Adh. Susp. Adh. Susp. IB: FAK PaxillinpoY118 GAPDH siFAK: -- + -- + -- + -- + D B 1.75 1.50 1.25 Colony-forming frequency (%) 1.00 Normalized colony formation 0.75 0.50 0.25 0 HT-25 RKO

More Related