1 / 15

Urine Culture

Urine Culture. Urine Gram Stain. Urine Culture Quantitation. Clean catch or catheterized urine Plate = 1000 organisms/ml Clean catch: >10 5 orgs/ml Suprapubic urine Any colony is significiant. Urine Cultures. 1. Plates initially read at 18-24 hr

gonzalezjose
Télécharger la présentation

Urine Culture

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Urine Culture

  2. Urine Gram Stain

  3. Urine Culture Quantitation Clean catch or catheterized urine Plate = 1000 organisms/ml Clean catch: >105 orgs/ml Suprapubic urine Any colony is significiant

  4. Urine Cultures 1. Plates initially read at 18-24 hr 2. All specimens plated after NOON of the previous day, hold another overnight 3. Gram positives take longer to grow

  5. Most Common Pathogens of Human Urinary Tract • Community acquired • E. coli is most frequent pathogen isolated • Klebsiella sp and other Enterobacteriaceae • Staphylococcus saprophyticus • Hospital acquired • E. coli, Klebsiella, otherEnterobacteriaceae • Pseudomonas aeruginosa • Enterococci and Staphylococci

  6. Abbreviated Identification • E. coli • Non-swarming, spot indole pos, oxidaseneg • Lactose positive (MacConkey or eosin methylene blue), PYR (pyrrolidonylarylamidase) test positive 2. API

  7. Abbreviated Identification • Proteus spp. • Swarming growth • Indole • Negative: P. mirabilis/penneri • P. mirabilis: maltose neg, ornithine pos • P. penneri: maltose pos, ornithineneg • Positive: P. vulgaris

  8. Abbreviated Identification • P. aeruginosa • Oxidase-positive bacillus • Typical smell (grapes) • Colony morphology P. aeruginosa: metallic/pearlescent, rough, pigmented, mucoid • Indole-negative

  9. Abbreviated Identification • CHROMagar Orientation • Presumptive ID for some UTI pathogens • E. coli (dark rose to pink) • Enterococci (turquoise blue) • S. saprophyticus(light pink to rose) • S. agalactiae(light blue-green to light blue) • Proteus-Morganella-Providencia group (brown) • Klebsiella-Enterobacter-Serratia group (dark blue) • Issues and challenges • All except E. coli and enterococci require further ID • Small E. coli colonies require spot indole • Poor growth of some gram-positive bacteria • Nonselective– other pathogens may or may not produce color change

  10. Abbreviated Identification • Enterococcus spp. • Cocci or coccobacilli in pairs and chains • >1 mm colonies • Non-hemolytic on SBA • Catalase-negative • PYR-positive (pyrrolidonyl-a-naphthylamide hydrolysis)

  11. Abbreviated Identification • S. agalactiae (GBS) • Cocci in pairs and chains • Catalase-negative • Narrow zone of beta-hemolysis on SBA • Rapid hippurate hydrolysis test (beta strep only) OR • Test for CAMP factor (spot or O/N) OR • Typing by particle agglutination • R/O beta hemolytic Enterococcus (PYR+) b-hem Enterococcus

  12. Abbreviated Identification • Yeast • Candida albicans • Microscopy required: oval, budding yeast • Colonies <48 h old on blood-containing medium with “feet” or mycelial projections *CHROMagar

  13. Abbreviated Identification • Candida CHROMagar

  14. Why females has higher incidence of UTI?? • Short urethra • Anus nearby urethra • contraceptives

  15. Now to the lab…

More Related