M.Sc . Andrés Ramírez Restrepo

1. VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA M.Sc. Andrés Ramírez Restrepo

2. INDEX CONTEXT 1 METHODS & MATERIALS 2 RESULTS & DISCUSSION 3 CONCLUDING REMARKS 4 WHAT’S NEXT? 5 BIBLIOGRAPHY 6 ACKNOWLEDGEMENTS 7

3. CONTEXT

4. 1.1.0 COLOMBIA https://www.muralesyvinilos.com/fotomurales/mapas/mapamundi-colores VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 4

5. 1.2.0 COLOMBIAN PRODUCTS OIL COAL GOLD EMERALDS FLOWERS COFFEE FOODS www.colombiatrade.com.co www.colombia.travel VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 5

6. 1.3.0 REGULATIONS To updatethisresolution, AgricultureMinistry and Social ProtectionMinistrywill do a yearlyupdate of theMRLsaccording to Codex Alimentarius. VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 6

7. 1.4.0 PROBLEM Does Colombia has knowledge, equipment and know-how available to surveythepresence of pesticideresidues in foodsaccording to Resolution2906 of 2007? How do we combine allourcapabilities to improvethem and demonstratethevalidity of ourmonitoringresults? Willtheinformationgatheredallowthefurtherissue of new and suitableMRLs in our country? VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 7

8. 1.5.0 JUSTIFICATION PRACTICAL METHOD REGULATORY VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 8

9. METHODS & MATERIALS

10. 2.1.0 VALIDATION ISO/IEC 17025:2005 «Confirmation by examination and the provision of objective evidence that the particular requirements for a specific intended use are fulfilled». International StandardsOrganization VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 10

11. 2.1.1 IMPLICATIONS VALIDATION AOAC 2007.01 GuideCG 4 ISO/IEC 17025 EN 15662 12571/2013 R-AC-01 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 11

12. 2.1.2 CRITERIA VALIDATION SANCO/12571/2013 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 12

13. 2.2.0 FOODS Sugarcane Saccharumofficinarum Lettuce Lactuca sativa Potato Solanumtuberosum Goldenberry Physalis peruviana Tomato Solanumlycopersicum Tamarillo Solanumbetaceum VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 13

14. Courtesy of M. Anastassiades 14

15. 2.3.0 GDCON https://www.museodeantioquia.co/noticia/jornadas-para-reflexionar-el-centro-de-medellin/ VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 15

16. 2.4.0 INSTRUMENTS GC Agilent Technologies 7890A VL MSD Agilent Technologies 5975C Aquity UPLC H Class XEVO TQD Waters VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 16

17. 2.5.0 RISK ISO/IEC 31000:2009 «Effect of uncertainty on objectives». International StandardsOrganization VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 17

18. 2.5.1 SURVEY RISK http://www.uxabilidad.com/recursos/mapa-politico-de-colombia-en-vectores.html VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 18

19. 2.5.1 SURVEY RISK http://pharus.webnode.es/turismo-sostenible/ VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 19

20. RESULTS & DISCUSSION

21. 3.1.0 EXTRACTS VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 21

22. 3.2.1 SELECTIVITY EVIDENCE VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 22

23. 3.2.2 MATRIX EFFECT EVIDENCE Y AXIS X AXIS VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 23

24. 3.2.3 PRECISION EVIDENCE VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 24

25. 3.2.4 TRUENESS EVIDENCE VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 25

26. 3.2.5 UNCERTAINTY EVIDENCE VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 26

27. 3.3.0 VALIDATION SUMMARY VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 27

28. 3.4.0 RISK MAP Significantchronictoxicityrisk in tomatoes! VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 28

29. 3.5.0 ACCREDITATION 13-LAB-053 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 29

30. CONCLUDING REMARKS

31. 4.0.0 CONCLUDING REMARKS Bymeans of QuEChERS and chromatographictechniques(GC-MS and UPLC-MS/MS) itwaspossible to provideobjectiveevidence of thefullfilment of therequirements. Thereis a gap between local and international know-how. Its causes should be found and attended to improveColombianinstalledcapacity. Thesurveysuggeststhatrisksfrompesticidesshould be attended. VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 31

32. WHAT’S NEXT?

33. 5.0.0 WHAT’S NEXT? More pesticides, more foods, more riskassessment. Nationalscalesurvey. UpdateColombianResolution. VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 33

34. BIBLIOGRAPHY

35. 6.0.0 BIBLIOGRAPHY x37 x20 Anastassiades, Lehotay, et al. x20 x19 EURLsDatapool ISO Res. 2906 VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 35

36. ACKNOWLEDGEMENTS

37. 7.0.0 ACKNOWLEDGEMENTS VALIDATION, ACCREDITATION, MONITORING AND RISK ASSESSMENT OF PESTICIDE RESIDUES IN FOODS FROM EASTERN ANTIOQUIA – Andrés Ramírez Restrepo 37

38. ANDRÉS RAMÍREZ RESTREPO Calle 62 No. 52-59 Torre 2. Laboratorio 232 Sede de Investigación Universitaria – SIU Universidad de Antioquia. Medellín, Antioquia quimico.andres@gmail.com Tel. +57 4 2630791 – 57 4 2196571 +57 300 567 4727

39. THANK YOU FOR YOUR KIND ATTENTION ¡MUCHAS GRACIAS POR SU ATENCIÓN!