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Comparative Analysis of Transcript Probing in Various Tissues Using Microarray and Q-PCR Techniques

This study examines the transcriptional profiles of different plant tissues across various developmental stages. Tissues sampled include mesocotyl, caryopsis, endosperm, embryo, bracts, radicle, roots, leaves, crowns, coleoptiles, anthers, pistils, scutellum, and stems, utilizing robust methodologies such as microarray and quantitative PCR (Q-PCR). The cultivars analyzed are 'Sloop' for Q-PCR and 'Morex' for microarray assessments, providing insights into tissue-specific gene expression and developmental biology.

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Comparative Analysis of Transcript Probing in Various Tissues Using Microarray and Q-PCR Techniques

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  1. Transcripts probed by microarray Transcripts probed by Q-PCR B A C ~ 22 000 ~ 17 ~ 59 Tissues probed by Q-PCR (cultivar ‘Sloop’) Tissues probed by microarray (cultivar ‘Morex’) B C A Mesocotyl (2 dgs) Caryopsis (14-16 dap) Endosperm (22 dap) Embryo (22 dap) Bracts (2 dba) Caryopsis (8-10 dap) Radicle (2dgs) Root (s) Leaf (s) Crown (s) Caryopsis (3-5 dap) Coleoptile (2dgs) Anther (2 dba) Pistil (2 dba) Scutellum (1 dgs) Stem Grain mid (10-13 dap) Root tip (10 dgs) Root mid zone (10 dgs) Leaf tip (10 dgs) Leaf base (10 dgs) Grain early (3-5 dap) Coleoptile (3 dgs) Floral (anthesis) Inflorescence Floral (ba)

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