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Nano-Bio-PhotoVoltaic Solid State Devices

Bottom-up NanoBiotechnology: from Membrane proteins to BioSolar Power. Nano-Bio-PhotoVoltaic Solid State Devices. Andreas Mershin Center For Biomedical Engineering. Lab for Molecular Self Assembly mershin@mit.edu , cookb@mit.edu http://web.mit.edu/lms/www/. October 12 th 2006

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Nano-Bio-PhotoVoltaic Solid State Devices

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  1. Bottom-up NanoBiotechnology: from Membrane proteins to BioSolar Power Nano-Bio-PhotoVoltaic Solid State Devices Andreas Mershin Center For Biomedical Engineering Lab for Molecular Self Assembly mershin@mit.edu, cookb@mit.edu http://web.mit.edu/lms/www/ October 12th 2006 Andreas Mershin, PhD & Brian Cook P.I. Shuguang Zhang, PhD Self-Assembling Peptides stabilize membrane proteins Dog on a chip

  2. Andreas Mershin Center For Biomedical Engineering Surfactant/detergent peptides can be designed to stabilize membrane proteins in unnatural environments such as bioelectronic circuits or sensors. Peptide stabilization can help in crystallization studies

  3. 1st Generation (flat monolayer) devices now in the Boston Museum of Science. Good concept but very low power and efficiency <1µm Nature, Boston Globe AP,Reuters, CNN, NYT, FT, BBC, etc. Biomimicry resonates with people

  4. Electric Nanoforest: ZnO nanowires are transparent, conducting tree-trunks Andreas Mershin Center For Biomedical Engineering ZnO transparent and conducting nanowires grown using CVD (high T) or wet deposition (low temp) High surface area, transparent, conducting, bio-friendly, enhancement factor ~200-2000

  5. IV Self-assembled “velcro” natural PsaD + + ZnO-PsaD ZnO-PS1 PS1 ITO ZnO PS-I ZnO-binding peptide sequence (GLHIPTGSSYSHR) ZnO NW ITO GLASS • Direct attachment • (less R, more I) Device architecture

  6. Olfactory receptors: smell background • The mammalian nose has the ability to rapidly distinguish between an enormous range of small molecules at low concentrations • The proteins responsible are olfactory receptors (ORs), a large class of sensory proteins which function combinatorially to allow the brain to discriminate odors • Currently, the molecular mechanism by which this receptor set functions is unknown

  7. Olfactory receptors: purification • Tagged and optimized OR genes are synthesized (PCR-based gene assembly) then inserted into “producer” cell lines • OR production is then “induced” in cell culture and the proteins harvested out and purified via the affinity tags (using HPLC) Olfactory receptors: structural & functional measurement • Structure: 2º confirmed via CD spectroscopy, 3º to be done with X-ray crystallography • Function: odorant binding via SPR (Biacore), plus other methods • Beyond: construction of prototype smell “biosensors” utilizing OR proteins

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