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Things to Do Today. Exam & Go over Exam. Throat Cultures Unknown gm (-) rod ID Assign HW: Fill in Table 1. Gram + ID. BAP Hemolytic Reactions. Used with permission. BAP Hemolytic Reactions. Alpha hemolysis. Beta hemolysis. gamma. KM. Perform throat Swab. 1 BAP per person
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Things to Do Today • Exam & Go over Exam. • Throat Cultures • Unknown gm (-) rod ID • Assign HW: Fill in Table 1. Gram + ID
BAP Hemolytic Reactions Used with permission
BAP Hemolytic Reactions Alpha hemolysis Beta hemolysis gamma KM
Perform throat Swab 1 BAP per person Swab first Quadrant and then smear swab on slide (throat) Use loop, flaming in between quadrants, and finish the 4 quadrant streak. Heat fix the slide throat plate
Gram (-) Test results Organism oxidase Urease MR H2S Gas Glucose Lactose Citrate Escherichia - - + - + + + - Pseudomonas + - - - - - - + Morganella - + - - + - - Proteus - + + + + + - - Enterobacter - - - - + + + + Salmonella - - - + + + - + Providencia - + - + + - - Unknown microbe
Oxidase Test Positive Negative
Urease Test Negative Positive KM
Methyl Red Test Negative Positive
KIA Slants uninoculated
KIA Slants uninoculated KM
Citrate test Negative positive KM
Gram Negative Unknown • Obtain unknown and write down number in your notes and on ALL media • Inoculate the MR and Urease broth tubes • Inoculate the Citrate agar by streaking its surface • Inoculate the KIA by streaking its surface AND then stabbing the butt.