1 / 13

Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells

Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells . Edgar Ferrer -Lorenzo, Nicole Gagnon, Anna Torre. 1. Optical microscopes have limitations. Diffraction limited Resolution depends on the wavelength of light and diameter of lens of optical microscope Specimen not alive

hija
Télécharger la présentation

Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Breaking the Diffraction Barrier:Super-Resolution Imaging of Cells Edgar Ferrer-Lorenzo, Nicole Gagnon, Anna Torre 1

  2. Optical microscopes have limitations • Diffraction limited • Resolution depends on the wavelength of light and diameter of lens of optical microscope • Specimen not alive The solution: STORM 2

  3. STORM fluorescence microscopy can overcome the diffraction limit A StORMimage is constructed from the localization of individual fluorescent molecules that are switched on and off using light of different colors 3

  4. STORM’s method improves the resolution of fluorescence microscopy 4

  5. STORM provides sub-diffraction-limit image resolution Rust et al., Nature Methods 3: 793-796 (2006). Betzig et al., Science 313: 1642-1645 (2006). 5

  6. Creating an image with STORM Huang et al., Cell 143: 1047-1058 (2010). 6

  7. Applications of STORM • Cell biology • Microbiology • Neurobiology 7

  8. STORM can resolve DNA structure Fluorophores bound to DNA fragment RecAcoated circular plasmid DNA 8 Rust et al., Nature Methods 3: 793-796 (2006).

  9. High resolution images of microtubules Bates et al., Science 317, 1749-1753 (2007). 9

  10. STORM can resolve 3D structure Bates et al., Science 317, 1749-1753 (2007). 10

  11. High resolution imaging of the action cytoskeletal network in neurons Xu et al., Science 339: 452-456 (2013). 11

  12. STORM has advanced biology • Unambiguous identification of specific proteins • Protein-protein interactions • Structure of small-type protein complexes • Live cell dynamics • Single molecule tracking • Cluster analysis and molecular counting 12

  13. Conclusions • STORM has found a clever way to get around the diffraction limit of resolution • Imaging resolution down to 20 nm • High-resolution live-cell imaging, thus enabling discovery of internal causes or origins of processes • All with visible light! • Imaging speed can be improved 13

More Related