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Myxobacteria movement Cell Tracking

Myxobacteria movement Cell Tracking. Tanya Kazakova University of Notre Dame Advisor: Dr. Mark Alber. About Myxobacteria. The myxobacteria are Gram-negative organisms (0.5 x 5-7 mm rods) that are capable of multicellular, social behavior.

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Myxobacteria movement Cell Tracking

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  1. Myxobacteria movementCell Tracking Tanya Kazakova University of Notre Dame Advisor: Dr. Mark Alber

  2. About Myxobacteria The myxobacteria are Gram-negative organisms (0.5 x 5-7 mm rods) that are capable of multicellular, social behavior. In the presence of nutrients, swarms of myxobacteria feed cooperatively. If the food supply is limited, the myxobacteria form aggregates called “fruiting bodies.”

  3. Developmental Cycle for Myxobacteria COUPLING CELL MOVEMENT TO MULTICELLULAR DEVELOPMENT IN MYXOBACTERIA by Dale Kaiser Nature Reviews Microbiology1, 45-54 (2003)

  4. Two engines of motility: A and S Adventurous Social A+S+ A-S+ A+S- Cells move alone Cells move as a group A-S- cells are unable to swarm or form fruiting bodies.

  5. Two engines of motility (cont) At any instant, only one end secretes slime and only one end assembles pili. One end cannot do both at the same time.

  6. Scenario Suppose we have an image produced to exhibit the results of a biology experiment. In most cases, we will have a black and white drawing such as one exhibited on the left.

  7. Goals General: Get the most information from the image Specific: • Track individual cells • Make cell density more obvious • Show the boundaries of expanding front • Get rid of noise

  8. About Scion Image National Institutes of Health Package http://rsb.info.nih.gov/nih-image/

  9. Using SI: Options -> Color Tables 8 Grays 8 Grays->Fire 1 Original Image Fire 1

  10. Example

  11. Cell Tracking Features

  12. MATLAB

  13. Example

  14. Possible Directions for Future Work

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