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Supplementary Figure 1; Large Transposon TnT

Supplementary Figure 1; Large Transposon TnT. E. E. E. ITR. ITR. CCTG 300. Probe. KT2/HSA-CCTG 300 9070 bp. + CpG. - CpG. Supplementary Figure 2; Rat genotype and transgene expression. 300 bp. 200 bp. A. ITR. ITR. E. P. BSD:GFP. miR30- Rab38. pA.

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Supplementary Figure 1; Large Transposon TnT

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  1. Supplementary Figure 1; Large Transposon TnT E E E ITR ITR CCTG300 Probe KT2/HSA-CCTG300 9070 bp + CpG - CpG

  2. Supplementary Figure 2; Rat genotype and transgene expression 300 bp 200 bp A ITR ITR E P BSD:GFP miR30-Rab38 pA KT2/KDRab38 3534 bp B 501 502 503 504 505 506 507 508 509 510 511 C L

  3. Supplementary Figure 3; F0 methylation 100 80 60 Percent CpG’s Methylated 40 20 0

  4. Supplementary Figure 4; LM-PCR founder 106 106 4.8 kb 2.7 kb

  5. Supplementary Figure 5; Insert copy number distribution A # Founders Copy number B # Founders Copy number

  6. Supplementary Figure 6; Broad transgene expression range in T2/shP2-mCFTR transgenic animals A Small Intestine Puro mRNA Non-methylated B Liver shCFTR1 Non-methylated

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