1 / 3

Species: Leptodea fragilis (wetlands/river mouths)

Genetic Sampling. Species: Leptodea fragilis (wetlands/river mouths) Pyganodon grandis (wetlands/ nearshore/tribs – Lake Erie) Quadrula quadrula (wetlands/river mouths – Lake Erie) Lampsilis siliquoidea/radiata (Lake St. Clair drainage only)

ingrid
Télécharger la présentation

Species: Leptodea fragilis (wetlands/river mouths)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Genetic Sampling • Species: • Leptodeafragilis (wetlands/river mouths) • Pyganodongrandis (wetlands/nearshore/tribs – Lake Erie) • Quadrulaquadrula (wetlands/river mouths – Lake Erie) • Lampsilissiliquoidea/radiata (Lake St. Clair drainage only) • Ligumianasuta (where it can be found 1-2 swab samples/refuge) • Numbers (site = refuge scale): • Microsats – clips only: • Q. quadrula(at least 10, but <50/site) • L. fragilis(at least 10, but <50/site) • L. siliquoidea(at least 10, but <50/site) • mtDNA (m/f lineage) – sacrifice animals • P. grandis (15 males/site) • L. fragilis (15 males/site, subsampled from above) – juveniles? • Swabs only (endangered species) • L. nasuta (1-2 swabs/site)

  2. Clipping instructions (more at methods meeting): • pry open shell with blunt end of scalpel/spatula (no blade!) • use thumb or end of pencil or small rubber stopper to wedge open shell • clip ~0.5 cm2 piece of mantle tissue from shell edge using surgical scissors (from basic dissection kit – keep them sharp) • clip from ventral-anterior side (avoid siphons) • Swab samples • as above, but sterile swab 2-3 times along foot – avoid touch mantle and siphons

  3. Logistics • Zanatta lab: • 2 grad students + 2 undergrads • 2 teams will join field teams as dedicated clippers/swabbers • Krebs lab: • Trevor Prescott + undergrad will assist in clipping • 3 genetics teams to assist other with general field work and will do clipping in field

More Related