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Effect of new cytokinin derivates application on Arabidopsis thaliana growth and development

Effect of new cytokinin derivates application on Arabidopsis thaliana growth and development. Jana Klásková Laboratory of Growth Regulators. Cytokinin metabolism. Cytokinin degradation. I I IN hibitors of CY tokinin DE gradation. LGR-2679. LGR-2678. Zatloukal et al., 2008.

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Effect of new cytokinin derivates application on Arabidopsis thaliana growth and development

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  1. Effect of new cytokinin derivates application on Arabidopsis thaliana growth and development Jana Klásková Laboratory of Growth Regulators

  2. Cytokinin metabolism

  3. Cytokinin degradation

  4. IIINhibitors of CYtokinin DEgradation LGR-2679 LGR-2678 Zatloukal et al., 2008

  5. Study of inhibitors in biological assays CKX assay

  6. Study of inhibitors in biological assays II Receptor assay (AHK3) Receptor assay (CRE1/AHK4)

  7. Study of inhibitors in biological assays III ARR5:GUS assay

  8. Effect of INCYDE-(F) application by spraying 10uM INCYDE-F 10uM INCYDE CONTROL 10uM INCYDE-F 10uM INCYDE CONTROL 10uM INCYDE-F CONTROL

  9. Effect of INCYDE-F applicationby spraying 1 uM 5 uM 10 uM CONTROL CONTROL CONTROL • Low temperature (15°C) for 4 weeks

  10. Effect of LGR-2679 application by drain LGR 2679; 0,1uM CONTROL 0,1uM X 1x 0,1uM X 3x 0,1uM X 5x 0,1uM X 1x 0,1uM X 3x 0,1uM X 5x 1x 3x 5x CONTROL CONTROL LGR 2679; 1uM CONTROL 1uM X 1x 1uM X 1x 1uM X 3x 1uM X 3x 1uM X 5x 1uM X 5x 1x 3x 5x CONTROL CONTROL

  11. Effect of LGR-2679 application by drain LGR 2679; 0,1uM CONTROL 0,1uM X 3x 0,1uM X 3x 0,1uM X 5x 0,1uM X 5x 0,1uM X 8x 0,1uM X 8x CONTROL 3x 5x 8x CONTROL CONTROL LGR 2679; 1uM 0,1uM X 8x 1uM X 3x 0,1uM X 3x 1uM X 5x 0,1uM X 5x 1uM X 8x CONTROL 3x 5x 8x CONTROL

  12. Mgr. Lukáš Spíchal, Ph.D. Mgr. Markéta Gemrotová, Ph.D Mgr. Jaroslav Nisler, Ph.D Mgr. Ota Blahoušek Thank you for your attention

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