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PEG protocol

PEG protocol

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PEG protocol

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  1. PEG protocol as of April 2005

  2. Cleaning, 2 hrs Step A (Slides) Step B (Coverslips) Step C (Reaction containers) Step D, 1hr (Aminosilanization) Step E, 2hr incubation (PEGylation)

  3. Notes • Reaction containers: any glass containers which are used for aminosilanization. We recycle them. We use a couple of glass staining dishes and a flask. • Aminosilane: obtained from UCT, Cat. # A0700, 25mg, expire after six months • PEG: obtained from Nektar Therapeutics. biotin-PEG (0H4M0H02, BIO-NHS-5000), mPEG (2M4M0H01, M-SPA-5000) • Methanol: ACS grade • The other chemicals such as sodium bicarbonate: from Fisher • The diameter of our centrifuge is 5”. The power of our sonicator is 130W.