1 / 27

Chapter 5

Chapter 5. Enzymes. Enzymes as organic catalysts. Enzyme is for lowering of activation energy. No. of substrate particles. Energy level of substrate particles. E. E’. No. of substrate particles. E: Activation energy when enzyme is not added. E’:Activation energy when enzyme is added.

kalil
Télécharger la présentation

Chapter 5

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Chapter 5 Enzymes

  2. Enzymes as organic catalysts • Enzyme is for lowering of activation energy.

  3. No. of substrate particles Energy level of substrate particles

  4. E E’ No. of substrate particles E: Activation energy when enzyme is not added. E’:Activation energy when enzyme is added. Energy level of substrate particles

  5. Lock and key hypothesis Active site substrate Products Enzyme-substrate complex Enzyme

  6. Induced fit model The active site is induced by the substrate to change its shape to fit the shape of the substrate.

  7. Enzyme can catalyze both forward and backward reactions. It does not alter the equilibrium position of a biochemical reaction. The normal progress of a biochemical reaction is due to the immediate removal of the end product.

  8. Specificity The degree of specificity varies from one enzyme to another. Intracellular enzyme works on one particular substrate. Extracellular enzyme works on a range of related substrates.

  9. Factors affecting enzymatic activity

  10. Temperature affects the energy content and hence the mobility of the enzyme and substrate molecules. Higher temperature enables enzyme and substrate molecules to collide more frequently and therefore facilitates the substrate molecule to bind with the active site of the enzyme. If the temperature is too high (above optimum), the polypeptide chains made up the enzyme will vibrate too much so that the structure will be disrupted, and the enzyme is said to be denatured.

  11. Temperature

  12. Temperature coefficient Q10 Q10 = Reaction rate at (X+10)oC Reaction rate at XoC When the temperature is below the optimum temperature, Q10 usually is 2. When the temperature is above the optimum temperature, Q10 usually is less than 1.

  13. T1 T2 No. of substrate particles T2>T1 Energy level of substrate particles

  14. pH Changes in pH alter the ionic charge on the enzyme surface, thus causing change in shape of its active site, thus diminishing its catalytic activity. If extremes of pH is encountered, the enzyme is denatured and loses its catalytic function.

  15. Reversible inhibitors: The effect is temporary. The inhibiting effect can be eliminated if the reversible inhibitor is removed. Irreversible inhibitors: This kind of inhibitor disrupts the bonds of the polypeptides which form the enzyme. Therefore the shape of the active site is altered permanently and loses its catalytic properties.

  16. Competitive Inhibitor Normal substrate Enzyme Reversible inhibitor(I): Competitive Inhibitor

  17. Usually the end product of a biochemical reaction acts as competitive inhibitor. This is known as end-product inhibition. Immediate removal of the end product facilitates enzyme reaction.

  18. Allosteric site The shape of the enzyme changes so that the normal substrate cannot bind to it. Normal substrate Non-competitive Inhibitor Non-competitive Inhibitor

  19. Example of competitive inhibitor Sulphonamides have very similar molecular shape to amino­benzoic acid, a compound essential for growth of many pathogenic bacteria. Therefore, sulphonamide acts as competitive inhibitor to check the growth of many bacteria in treatment of many diseases caused by bacteria.

  20. Example of non-competitive inhibitor Diisopropylflurophosphate (DFP), a nerve gas used in warfare is a non‑competitive inhibitor. DFP acts on enzyme cholinesterase which is involved in synaptic transmission. DFP causes prolonged muscle contraction and death is the end result.

  21. Enzyme concentration and substrate concentration affects the concentration of the enzyme-substrate complex and hence the rate of enzyme-catalysed biochemical reaction.

  22. Enzyme cofactorIt is non-protein substance which makes some enzyme to function more efficiently.

  23. Enzyme activator • It is an inorganic ion which loosely bound to an enzyme or an substrate to increase the chance to an enzyme-substrate complex forming. Some times, the inorganic ions are free and does not bind to the enzyme or substrate, • e.g. copper, iron, calcium, Cl- etc.

  24. Coenzyme • It is an organic non-protein molecule firmly associated with the enzyme and is essential for enzyme activity. • It acts a carrier for transferring chemical groups or atoms from one enzyme to another. • Many coenzymes are synthesized from vitamins. • e.g. NAD

  25. Prosthetic group • It is an organic non-protein group which is tightly bound to the enzyme or as an integral part with the enzyme. • It can act as carriers of atoms or electrons while transferring one compound to another in an overall metabolic pathway. • e.g. FAD, haemoglobin etc.

  26. Application of enzymes Some washing powders are biologically active. They contain enzyme protease to digest the protein dirt. Meat tenderizer contains enzyme protease which break down and soften the texture of meat.

More Related