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Interaction Analysis of HIF-1α and HIF-2α with SIRT1 in SiHa Cells

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This supplementary figure presents the results of immunoprecipitation (IP) experiments done in SiHa cells expressing wild-type SIRT1 and its H363Y mutant. The analysis focuses on the acetylation levels of HIF-1α and HIF-2α in relation to SIRT1 activity. Control conditions are also included, assessing the inputs of actin as a loading control. The findings contribute to the understanding of SIRT1's role in regulating hypoxia-inducible factors and their acetylation status in cervical cancer cells.

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Interaction Analysis of HIF-1α and HIF-2α with SIRT1 in SiHa Cells

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  1. Supplementary Figure S4 SiHa/7.4 SiHa/6.5 EX-527 A B EX-527 ctrl ctrl IP: Ac-Lys HIF-1α HIF-1α Ac-Lys HIF-2α HIF-2α SIRT1 inputs actin C D E SiHa/7.4 SiHa/6.5 Flag-SIRT1 wild-type - + - - + - Flag-SIRT1 H363Y - - + - - + IP: Ac-Lys HIF-1α HIF-1α Ac-Lys HIF-2α HIF-2α SIRT1 inputs Flag actin

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