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Impact of SI-34 and Etoposide on Apoptosis in Cell Culture

This study examines the effects of SI-34 and Etoposide on apoptosis in cell culture over various time points, analyzing caspase-3 activity and Annexin V staining. Results indicate differential responses between treated and untreated cultures.

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Impact of SI-34 and Etoposide on Apoptosis in Cell Culture

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  1. ctrl SI 34 1 μM % of cells % of cells SI 34 10 μM etoposide 50 μM annexin V annexin V untreated culture 9.1 % SI 34 1 µM 72 h 38.9 % SI 34 10 µM 72 h 43.8 % etoposide 50 µM 72 h 48.5 % no treated SI 34 1 µM SI 34 10 µM A B 200 **° ^ ** 24 hours 48 hours 72 hours 150 caspase 3 activity (% untreated cultures) FL-1 FL-1 FL-1 100 % of cells 50 0 24 48 72 time (hours) annexin V SUBSET NAME FL1-H SUBSET NAME FL1-H SUBSET NAME FL1-H untreated culture 8.1 % untreated culture 8.6 % SI 34 1 µM 24 h 9.9 % SI 34 1 µM 48 h 18.1 % SI 34 10 µM 24 h 12.1 % SI 34 10 µM 48 h 28.3 % etoposide 50 µM 24 h 26.5 % etoposide 50 µM 48 h 37.3 % C

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