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Epitope Tagging:

Epitope Tagging:. Yak1: Protein: N- Met-Asn-Gln-…-Thr-Ser-C The HA epitope: Protein: N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C. Yak1. Yak1. Epitope Tagging:. Yak1: DNA: AAAG ATG AATCAG ... ACATCG TGA CCTA... Protein: N- Met-Asn-Gln-…-Thr-Ser-C The HA epitope:

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Epitope Tagging:

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  1. Epitope Tagging: Yak1: Protein: N-Met-Asn-Gln-…-Thr-Ser-C The HA epitope: Protein: N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C Yak1 Yak1

  2. Epitope Tagging: Yak1: DNA: AAAGATGAATCAG ... ACATCGTGACCTA... Protein: N-Met-Asn-Gln-…-Thr-Ser-C The HA epitope: DNA: TATCCCTACGACGTTCCGGATTATGCC Protein: N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C Yak1

  3. Epitope Tagging: Yak1: DNA: AAAGATGAATCAG ... ACATCGTGACCTA... Protein: N-Met-Asn-Gln-…-Thr-Ser-C The HA epitope: DNA: TATCCCTACGACGTTCCGGATTATGCC Protein: N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C Fusion Gene: AAAGATGTATCCCTACGACGTTCCGGATTATGCC AATCAG… Yak1

  4. Epitope Tagging: Yak1: DNA: AAAGATGAATCAG ... ACATCGTGACCTA... Protein: N-Met-Asn-Gln-…-Thr-Ser-C The HA epitope: DNA: TATCCCTACGACGTTCCGGATTATGCC Protein: N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C Fusion Gene: AAAGATGTATCCCTACGACGTTCCGGATTATGCC AATCAG... Fusion Protein: N-Met-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-Asn-Gln-…-Thr-Ser-C Yak1 Yak1

  5. Making a Fusion Protein: Yak1: DNA: AAAGATGAATCAG ... ACATCGTGACCTA... Protein: N-Met-Asn-Gln-…-Thr-Ser-C GFP: DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC... Protein: N-Met-Ser-Lys-… -Leu-Lys-C Yak1 GFP

  6. Making a Fusion Protein: • Yak1 Gene: • DNA: AAAGATGAATCAG ... ACATCGTGACCTA... • GFP Gene: • DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC... • Fusion Gene: • AAAGATGAATCAG ... ACATCGATGAGTAAA ... CTGAAATAATGACCTA...

  7. Making a Fusion Protein: Yak1 Gene: DNA: AAAGATGAATCAG ... ACATCGTGACCTA... Protein: N-Met-Asn-Gln-…-Thr-Ser-C GFP Gene: DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC... Protein: N-Met-Ser-Lys-… -Leu-Lys-C Yak1 GFP N C Fusion Gene: AAAGATGAATCAG ... ACATCGATGAGTAAA ... CTGAAATAATGACCTA... Fusion Protein: N-Met-Asn-Gln-…-Thr-Ser-Met-Ser-Lys-… -Leu-Lys-C

  8. Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes

  9. GFP-RAS2 in living Saccharomyces cerevisiae

  10. Images from Roger Tsein’s Lab

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