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Primers for Examining Gene Expression and Amplifying a-DOX and LOX Promoters

This document outlines the sets of primers utilized to investigate gene expression and to amplify the promoters of a-DOX and LOX genes. Section A details the primers used for semi-quantitative RT-PCR to analyze gene expression levels. Section B lists the primers designed specifically for the amplification of a-DOX and LOX promoters. Section C describes the primers used to examine the expression of LOX1 and LOX5 via RT-PCR in both wild type and T-DNA insertion lines, enabling comprehensive analysis of these genes.

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Primers for Examining Gene Expression and Amplifying a-DOX and LOX Promoters

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  1. Table 2 Sets of primers used to examine gene expression and to amplify promoters of a-DOX and LOX genes (A) Sets of primers used to examine gene expression by semiquantitative RT-PCR (B) Sets of primers used for amplification of the a-DOX and LOX promoters (C) Sets of primers used to examine LOX1 and LOX5 expression by RT-PCR in wild type and T-DNA insertion lines.

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