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Phylogenetic Analysis of VvKu70 Protein and its Association with Close Ku70 Proteins

This study compares the amino acid sequences of Ku70 proteins from various plant species, highlighting the evolutionary relationships and structural features. The phylogenetic tree was constructed using Clustal W and MEGA 5 software. The alignment of VvKu70 with related proteins reveals conserved regions and functional domains. Copy number analysis shows VvKu70 as a single-copy gene in the grapevine genome, determined by Southern blot analysis.

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Phylogenetic Analysis of VvKu70 Protein and its Association with Close Ku70 Proteins

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  1. Fig.S1 Phylogenetic analysis of VvKu70 proteinin relation with other closely related Ku70 proteins. The amino acid sequences used to build up the phylogenetic tree were: PnKu70 (BAF03493.1), RcKu70 (XP_002521532.1), VvKu70 (XP_002267875.1), AtKu70 (NP_564012.1), MtKu70 (XP_003592861.1), VrKu70 (AAT48365.1), GmKu70 (XP_003556580.1), OsKu70 (BAC83190.1), HvKu70 (AEO86623.1) and BdKu70 (XP_003557466.1). The bootstrapped tree (with 1000 replicates) was constructed using Clustal W and MEGA 5 software.

  2. Ku70 core domain SAP domain Fig. S2 Alignment of VvKu70 (XP_002267875.1) protein sequence with sequences from Ricinuscommunis (XP_002521532.1), Vignaradiata (AAT48365.1) and Arabidopsis thaliana (NP_564012.1). Identical residues are black shaded and Ku70 core domain is red underlined. The SAP DNA binding domain is boxed in blue.

  3. NheINcoI XhoI 10kb 6kb 3kb Fig. S3 Copy number determination of VvKu70 in grapevine genome by southern blot analysis. The VvKu70 gene is present as a single copy number gene in Vitis vinifera cv. Mango genome. The genomic DNA was digested with NheI, NcoI and XhoI and probed by a probed against VvKu70 coding sequence. The approximate positions of bands are indicated.

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