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Supplemental Figure 1

Supplemental Figure 1

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Supplemental Figure 1

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  1. Supplemental Figure 1 A B panobinostat

  2. Supplemental Figure 2 Control 20 50 nM BC2059, 24 hr A OCI-AML3 B

  3. Supplemental Figure 2 Control 20 50 100 nM BC2059, 48 hr C OCI-AML3 TO-PRO-3 iodide Annexin V-FITC D Control 50 100 nM BC2059, 48 hr TO-PRO-3 iodide HL-60 Annexin V-FITC E Control 20 50 100 nM BC2059, 48 hr Primary FLT3-ITD AML Propidium iodide

  4. Supplemental Figure 3 OCI-AML3 MV4-11 C B A 0 20 50 100 nM, BC2059, 24 h 0 20 50 100 nM, BC2059, 24 h BIMEL BIMEL BIML BIML BIMS BIMS p27 p27 MCL1 β-Actin β-Actin D Primary FLT3-ITD AML 0 20 50 100 nM, BC2059, 24 h β-Catenin BIMEL BIML BIMS p27 β-Actin

  5. MV4-11 cells Supplemental Figure 4 Merge TBL1/β-Catenin A TBL1 DAPI β-Catenin Primary AML cells B CD34+ Primary FLT3-ITD AML cells TBL1 β Catenin Merge DAPI β-catenin DAPI Merge Control Control Control 20 nM BC2059 50 nM BC2059 20 nM BC2059 100 nM BC2059 D CD34+ FLT3 WT Primary AML cells 100 nM BC2059 C Merge DAPI β-catenin Control 100 nM BC2059, 16 hours

  6. Supplemental Figure 5 A B D C

  7. Supplemental Table 1: Genes with greater than 3 fold induction in OCI-AML3 cells following treatment with 100 nM of BC-2059 for 8 hours.

  8. Supplemental Table 2: Genes with greater than 2 fold depletion in OCI-AML3 cells following treatment with 100 nM of BC-2059 for 8 hours

  9. Supplemental Table 3: Ingenuity Pathway Analysis of the 4 most perturbed networks in OCI-AML3 cells following treatment with 100 nM of BC-2059 for 8 hours.

  10. Supplemental Figure 6 C OCI-AML3 BC2059 PS Fa CI (nM) (nM) 60 6 0.566 1.043 70 7 0.701 0.961 75 7.5 0.811 0.812 80 8 0.836 0.811 90 9 0.9 0.735 A HL-60 BC2059 PS Fa CI (nM) (nM) 60 6 0.52 1.027 70 7 0.66 0.792 75 7.5 0.771 0.578 80 8 0.756 0.653 90 9 0.867 0.442 MV4-11 BC2059 PS Fa CI (nM) (nM) 30 3 0.474 0.963 35 3.5 0.641 0.612 40 4 0.746 0.452 50 5 0.942 0.128 B * *

  11. Supplemental Figure 7 OCI-AML3 cells A TBL1 DAPI Merge Control 10 nM PS, 16 h 20 nM PS, 16 h 50 nM PS, 16h B OCI-AML3 0 10 20 50 nM, PS, 16 h TBL1 β-Actin

  12. Supplemental Figure 8 AML #1 AML #2