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Comprehensive Analysis of Sperm and Testes Utilizing H3K4me3 Histone Enrichment Techniques

This study presents a detailed examination of sperm and testes samples, focusing on histone methylation patterns, specifically H3K4me3 enrichment. Using supplemental figures, various sperm classifications (A-, A+, TOTAL) and testes analyses (MIR181c, Poly(A+)) are explored to identify significant differences in epigenetic modifications. Our findings offer insights into the functional implications of these histone marks in spermatogenesis and testicular health, enhancing our understanding of male reproductive biology.

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Comprehensive Analysis of Sperm and Testes Utilizing H3K4me3 Histone Enrichment Techniques

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  1. Supplemental Figure 1

  2. SPERM A- Sp_D62[A-] Sp_D62[A+] SPERM A+ Sp_D62[Tt] SPERM TOTAL Sp_D64[Tt] Sp_D66[Tt] Sp_P1[Tt] Sp_D62[SP] SPERM SPIA Sp_D64[SP] Sp_D66[SP] Sp_P2[SP] Te_PCl[SP] TESTES SPIA Te_PAm[A+] TESTES Poly (A+) Te_PCl[A+] PRM2 Supplemental Figure 2

  3. Supplemental Figure 3

  4. Sperm (A-) Sperm (A+) SpermTOTAL Testes MIR181c Supplemental Figure 4

  5. A H3K4me3 Histone Enriched HMR Sperm Testes HOX-A Cluster EVX1 B H3K4me3 Histone Enriched HMR Sperm Testes EVX1 Supplemental Figure 5 A,B

  6. C H3K4me3 Histone Enriched HMR Sperm Testes EVX2 HOX-D Cluster D H3K4me3 Histone Enriched HMR Sperm Testes EVX2 Supplemental Figure 5 C,D

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