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This study investigates the behavior of tumor cells in a controlled environment using a monolayer in the upper collagen layer over a 120 μm thickness. By analyzing the migration of these cells through the lower collagen gel layer, we aim to understand the mechanisms driving tumor cell invasiveness. The insights gained from this quantitative analysis can potentially inform therapeutic strategies for cancer treatment, particularly focusing on the role of the extracellular matrix in tumor progression.
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A Day 0 Day X Quantitative analysis Invasion 120 μm Upper collagen layer Tumor cells monolayer Origin of Migration Lower collagen gel layer A 120 μm