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Molecular Biology Quiz: Splicing and Phylogenetic Analysis

This quiz for Biol 302 covers key concepts in molecular biology, including alternative splicing and phylogenetic analysis of the UBX protein across multiple species. Students are required to complete sections on gene structure, identify functional regions such as the polyA tail, and analyze a protein alignment to create consensus sequences and evolutionary trees. The emphasis on conserved amino acids and structural regions offers a deeper understanding of molecular evolution and protein function.

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Molecular Biology Quiz: Splicing and Phylogenetic Analysis

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  1. Quiz 2: Biol 302 Spring2011 Where did you take Biol 100/102 or equivalent?: Where did you take Biol 202 or equivalent?: Name:_______________ Part 1: Complete gene with alternate splice Name all structures with black arrows or parenthessis (10pts- All or None) Where would you draw the polyA tail in the gene above?______________

  2. Part2 :Given the following protein alignment for part of the UBX protein from 8 species: Species: Yellow Region 5 2 3 4 1 A B C D E F G H _ _ _ _ _ Make Consensus for each underlined position (5pts: all or none) Based on these 5 residues draw phylogenetic tree for species A through H. (5pts: all or none) Based on Yellow domain draw phylogenetic tree for species A through H. (5pts: all or none) Homeodomain starts at first amino acid: Draw a line in the above alignment as to where conservation of amino acid function likely ends for all 8 species. HINT: it is right around the end of the Homeodomain. (Extra Credit: 1pt)

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