Design Project Introduction

1. Design Project Introduction Katie McAlindon and Tanya Khan

2. Agenda • Concept and Process Background • Report Content • Writing/Style • Grading • Sign up

3. Concept and Process Background

4. Concept • Your group is a company placing a bid to provide a unit operation in a process under construction • You will design the most appropriate unit given the conditions available in SuperPro • Must be a feasible design (therefore you should consult real companies and try to get real quotes etc) • Detailed report will be submitted at a professional standard

5. Project 1: β-galactosidase Production • an intracellular enzyme produced by Escherichia coli. • mainly used in the utilization of cheese whey. More specifically, • immobilized reactors convert lactose found in cheese whey to glucose and galactose, yielding a sweetened product • additive to ice cream, egg-nog, yogurt, and other dairy products • Another application of b-Gal is in the treatment of milk products. • produces lactose free milk products • This example analyzes a plant that produces 18,329 kg of b-Gal per year in 216 batches. PROCESS DESCRIPTION Fermentation Section • Fermentation media are prepared in a stainless steel tank (V-101), a continuous sterilizer (ST-101), a compressor (G-101) and an absolute air filter (AF-101). Primary Recovery Section • The first step of the primary recovery section is cell harvesting to reduce the volume of the broth and remove extracellular impurities; Purification Section • Next the product stream is purified by an ion exchange chromatography column (C-101) and then concentrated and further processed.

6. Project 2: Monoclonal Antibody Production • This example analyzes the production of a therapeutic monoclonal antibody using animal cell culture. The recipe analyzed produces 19 kg of purified product per batch. • Infliximab is a monoclonal antibody against TNF-α. • The SuperPro design is not specific to this antibody, but you can assume this is the antibody being produced for your project • Approced by the FDA for treatment of psoriasis, Crohn’s disease, rheumatoid arthritis among other autoimmune diseases • Works by binding to tumour necrosis factor alpha (a key part of the autoiummune reaction) • The exact mechanism is unknown • Cases programmed cell death of T-lymphocytes extressing TNF-α • A combination of a mouse and human antibody (artificial) (Chimeric monoclonal antibody) Upstream Processing • The upstream section is split in two sub-sections, the Inoculum preparation and the Bioreaction section. Downstream Processing • Bulk contaminants removed • Protein concentrated • Sterilized • Further processed to final product

7. Monoclonal Antibody Flow Sheet

8. Report

9. Our Expectations • Professionalism and industry standard work are of paramount importance Our recommended Flow: • Executive Summary (distilled up to the executive level, less technical content, important conclusions) • Tables of Contents and Figures • Introduction to the purpose of the project, and your specific unit • Discussion of your design • Conclusions and Recommendations

10. Executive Summary • Brief summary of main conclusions (like an abstract) • Written for the business world, not scientists • Should focus on economic conclusions and design • Sell your design • 1 page maximum

11. Introduction to your process/unit • What does your unit do? • How does it fit into the overall process? • Why is it needed? • Discuss flows in and out • Give a purpose to your proposal

12. Discussion of your Design • Pricing and sizing for appropriate capacity • Suppliers, quotes • Materials • Power requirements • Safety concerns (back ups, emergency shut off etc) • Environmental concerns • Waste streams (and destination of waste) • Human-power (automated vs manual operation) • Maintenance and cleaning • Inefficiencies or recommendations This is not just stating facts, but comparing and analyzing and proving that your design is the best

13. Conclusions and Recommendations • Do not just restate the executive summary • Can be more technical than ES. • Draw together all the talking points about your design and the conclusions that were made in each section (i.e. power, safety, environments, costs) • Provide recommendations

14. How to write/ Style Unprofessional reports will not get a high grade (A)

15. Main Body • Each section should have a clear purpose, discussion, and conclusion • Just a technical description is not enough, must explain why we care • Tangible and real equipment are important, but full quotes should be in an appendix and referenced in your main report. • Irrelevant material will be penalized for lack of professionalism • Grammar and tense should be correct • Consistency in style, format, voice

16. Figures and Appendices • Figures should have a fully descript caption to describe what we are seeing and how it is relevant • Incomprehensible figures will be penalized • Appendices • Flow of report can be maintained by referencing quotes in the appendix • Appendices must be referenced in the main body and relevant • Unnecessary appendices will be penalized

17. Style and Formatting • Should be at a level you would submit in industry

18. Grading

19. Marking Scheme Style (/20) • Includes professionalism, relevancy, grammar and typos, consistency Technical Content (/60) • All design factors considered, supplier found and recommended with good comparison and analysis, prove your design Conclusions (/20) • Should be able to be understood without reading the body • Should be obvious and succinct

20. Sign up

21. Sign up • 3 people per group, ONE group contact e-mail • BE LEGIBLE! • Location: Walter Light 714 • Time: To Be Discussed with you! • Units will be assigned Friday afternoon through e-mail • First come first serve on sign up list An email from us will include: • Your project SuperPro flow sheet and your assigned unit • A copy of this presentation • A SuperPro tutorial to do at your convenience